Evaluation of soluble CD40L in children with type 1 diabetes mellitus and its relation to diabetes associated vasculopathy | ||||
| The Egyptian Journal of Pediatric Allergy and Immunology | ||||
| Article 8, Volume 19, Issue 2, October 2021, Page 105-112 PDF (310.64 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejpa.2021.61225.1020 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mahmoud Hodeib  1; Mohamed meabed2; Khadiga Abougabal3; Ghada Etman4
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| 1Pediatrics, Beni-Suef university ,Beni Suef ,Egypt | ||||
| 2Pediatrics,Beni-Suef university, Beni Suef ,Egypt | ||||
| 3clinical and chemical pathology , Clinical and Chemical Pathology Department , Faculty of Medicine, Beni-Suef University, Beni Suef, Egypt; | ||||
| 4Pediatrics Department, Faculty of Medicine, Beni-Suef University, Beni Suef, Egypt | ||||
| Abstract | ||||
| Background: Type 1 diabetes Mellitus (T1DM) is a dynamic autoimmune disorder characterized by retrogressive insulin production as a consequence of autoimmune-mediated destruction of insulin producing pancreatic β-cells. This study aims at evaluating serum levels of soluble CD40 Ligand (sCD40L) as biomarker for early detection of complications associated with T1DM in children. Methods: A comparative cross-sectional study was conducted on 120 children with T1DM attending the Pediatric Diabetes Clinic of Beni-Suef University Hospital, in the period from April 2019 to January 2021. They were classified into 2 groups each with 60 patients, according to the presence or absence of microvascular diabetic complications. A group of 60 age and sex-matched healthy subjects were also included as a control group. Enrolled patients were subjected to detailed clinical evaluation, fundus examination, nerve conduction velocity, in addition to laboratory testing including fasting lipid profile, mean 2-hours postprandial blood glucose, Glycated hemoglobin (HbA1c), serum levels of sCD40L using enzyme linked immunosorbent assay (ELISA) and urinary albumin-to-creatinine ratio by an immuno-nephelometric method. Results: Patients’ group comprised 68 males (56.7%) and 52 females (43.3%). The most common complication encountered in the studied patients with T1DM was micro albuminuria in 44 patients (73.33%), followed by peripheral neuropathy in 18 (30%) and retinopathy in two (3.33%). Serum sCD40L levels were significantly elevated in patients with complicated T1DM (6956±2025 pg/ml) compared to non-complicated group (547.7±125.4 pg/ml) and healthy controls (205.7±55.3 pg/ml) [p < 0.001]. A cut-off level of sCD40L more than 315 pg/mL could discriminate complicated from non-complicated cases with 96 % sensitivity and 90 % specificity, area under the curve 0.96 (p < 0.001). Serum sCD40L levels were positively correlated with HbA1c and urinary albumin excretion (p < 0.001). Conclusion: Serum sCD40L could be a helpful biomarker for monitoring microvascular complications of T1DM. Wider scale longitudinal studies might help to assess the value of sCD40L in following-up diabetic patients and predicting complications so that preventive measures can be timely taken. | ||||
| Keywords | ||||
| Microalbuminuria; Soluble CD40 ligand; Type 1 Diabetes | ||||
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