Immobilization of β-galactosidase on Carrageenan Gel via bio-inspired Polydopamine Coating | ||||
| Journal of Textiles, Coloration and Polymer Science | ||||
| Article 8, Volume 15, Issue 1, December 2018, Page 85-93 PDF (1.56 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jtcps.2018.6267.1012 | ||||
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| Authors | ||||
Mohamed A Yassin   1; Mohamed Naguib2; Mona H Abdel Rehim3; Korany A Ali4
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| 11 Packing and Packaging Materials Department, National Research Centre, Giza, Egypt 2 Advanced Materials and Nanotechnology Lab., Center of Excellence, National Research Centre, Giza, Egypt | ||||
| 22 Advanced Materials and Nanotechnology Lab., Center of Excellence, National Research Centre, Giza, Egypt 3 Polymers and Pigments Department, National Research Centre, Giza, Egypt | ||||
| 31 Packing and Packaging Materials Department, National Research Centre, Giza, Egypt | ||||
| 42 Advanced Materials and Nanotechnology Lab., Center of Excellence, National Research Centre, Giza, Egypt 4 Applied Organic Chemistry Department, National Research Centre, Giza, Egypt | ||||
| Abstract | ||||
| In the context of diligent efforts directed to develop new polymeric matrices for enzyme immobilization, we developed carrageenan gel disks activated with bio-inspired polydopamine layer. The carrageenan gel disks were firstly treated with polycationic polymer (hyperbranched polyamidoamine) followed by a polydopamine layer. Such a layer can act as an active platform for the covalent immobilization of enzymes via Michael addition and Schiff base. The mechanical strength of the coated gel disks was obviously enhanced. β-galactosidase was successfully immobilized with a loading efficiency of about 52 %. Furthermore, the immobilized β-galactosidase exhibited enhanced activity at a broader range of pH and temperature. Additionally, the immobilized β-galactosidase preserved around 80 % of the initial activity during the eleven reusability cycles. Generally, activation of the gel disks with the bio-inspired polydopamine layer considered as a safe alternative to the glutaraldehyde due to its cytotoxic. Accordingly, the easily formed carrageenan gel disks coated with polydopamine could have potential as enzyme carrier in drug and food industries. | ||||
| Keywords | ||||
| Carrageenan; Dopamine; Immobilization; β-galactosidase; Lactose free | ||||
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