Evaluation of the effect of endodontic chelating solutions on push out bond strength of endodontic sealers (An in-vitro study) | ||||
Al-Azhar Dental Journal for Girls | ||||
Article 7, Volume 5, Issue 4, October 2018, Page 365-372 PDF (535.98 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/adjg.2018.20022 | ||||
View on SCiNiTO | ||||
Authors | ||||
Mai Mohamed Salah El-Din1; Mohsen Nour El-Din2; Mahmoud Hassan Mohamed3 | ||||
1Dentist at Ministry of Health, Egypt. | ||||
2Professor of Endodontics, Faculty of Dental Medicine for girls, Al-Azhar University. | ||||
3Professor and Head of Endodontic department, Faculty of Dental Medicine for Boys, Al Azhar University, Assuit branch. | ||||
Abstract | ||||
Aim: This study was carried out to evaluate the effect of various chelating solutions (EDTA-Apple Vinegar-Sodium Hypochlorite)on the radicular push-out bond strength of zinc oxide nanosealer and AH Plus resin based rootcanal sealer. Materials and Methods: 180 extracted single-rooted human teeth with complete roots, fully formed apices were used in this study,on completion of instrumentation, the specimens will be randomly divided into three groups (n=60) according to chelating solution applied: Group ( I ) 1.5 mL of 17% EDTA (pH = 7), Group ( II ) 2.5 mL of vinegar apple and Group ( III ) 2 mL of 5.25% sodium hypochlorite (NaOCl). These groups are subdivided into two subgroups (n=30) each according to root canal sealer used: Subgroup A: AH Plus and Subgroup B: Zinc oxide nanosealer. Then the specimens in the subgroups will be further randomly divided into two groups (n=15) according to storage time: Subgroup 1:one week storage time and Subgroup 2: one month storage time The root canals were prepared using ProTaper rotary instruments up to master apical rotary size F3 (#30), in conjunction with 2 mL of Normal saline solution (Sodium Chloride 0.9%) irrigation between each file and the chelating solution was applied for 1 minute then final rinse with distilled water. The sealers were loaded and introduced to the canal using K file size40.Testing push-out bond strength was done for all samples using the Intrson universal testing machine. Results: It was found that the highest push-out bond strength mean value recorded for (EDTA+ AH Plus) (1 week) group (IA1) (3.424 MPa) followed by (Apple Vinegar+ AH Plus) (1 week) group (IIA1) (1.263 MPa) while the lowest mean value recorded for (NaOCl+ZnO nanosealer) (1 month) group (IIIb2) (0.575 MPa). The difference between different groups was statistically significant as revealed with one way ANOVA test (p<0.05). Tukey’s post-hoc test showed non-significant (p>0.05) differences between {(Apple Vinegar+AH Plus) and (NaOCl +AH Plus) (1 week)}, {( EDTA+ AH Plus), (Apple Vinegar+ AH Plus) and(NaOCl+ AH Plus) (1 month)}, {(EDTA+ ZnO nanosealer) and (NaOCl+ ZnO nanosealer) (1 month)}. Conclusions: AH Plus sealer had the highest push-out bond strength when used with EDTA, ZnO nanosealer had the highest push –out when used with apple vinegar and all AH Plus subgroups had higher push-out bond strength than ZnO nanosealers subgroups | ||||
Keywords | ||||
AH Plus; ZnO nanosealer; Push-out bond strength | ||||
References | ||||
Grandini S, Balleri P, Ferrari M. Evaluation of Glyde File Prepin combination with sodium hypochlorite as a root canal irrigant. J Endod ,2002;28:300-3. 2- Scelza MF, Pierro VS, Chagas MA, Silva LE, Scelza P. Evaluation of inflammatory response of EDTA, EDTA-T, and citric acid in animal model. Int Endod J, 2010;36:515-9. 3- Eick JD, Wilko RA, Anderson CH, Sorensen SE. Scanning electron microscopy of cut tooth surfaces and identification of debris by use of the electron microprobe. J Dent Res ,1970;49:59–68. 4- Brännström M, Johnson G. Effects of various conditioners and cleaning agents on prepared dentin surfaces: a scanning electron microscopic investigation. J Prosth Dent., 1974; 31, 422–30. 5- Marending M, Paqué F, Fisher J, Zehnder M. Impact of irrigant sequence mechanical properties of human root dentin. J Endod. ,2007; 1325-1328-3 6- Sirtes G, Waltimo T, Schaetzle M, Zehnder M. The effects of temperature on sodium hypochlorite short-term stability, pulp dissolution capacity, and antimicrobial efficacy. J Endod. ,2005;31:669–671. 7- Torabinejad M, Handysides R, Khademi A, Bakland L. Clinical implications of the smear layer in endodontics: a review. Oral Surg Oral Med Oral Patho Oral Radio Endod., 2002; 94:658-66. 8- Shahravan A, Haghdoost AA, Adl A, Rahimi H, Shadifar F. Effect of smear layer on sealing ability of canal obturation: A systematic review and meta-analysis. J Endod, 2007;33:96-105. 9- Costa D, Dalmina F, Irala L. The use of the vinegar as a chemical auxiliary in endodontics: a literature review. Rev Sul-Bras Odontol. 2009; 6:185-193. 10- Estrela C, Lopes H, Elias C, Leles C, Pécora J. Surface cleaning of root canal by apple vinegar , sodium hypochlorite , chlorohixidine and EDTA. Rev Assoc Paul Cir Dent., 2007; 61:117-22 11- Estrela C, Estrela C, Cruz-Filho A, Pécora J. ESP substance: option in endodontic therapeutic. J Bras Endod,2005;5:273-9. 12- Spanó J, Silva R, Guedes D, Sousa-Neto M, Estrela C, Pécora J. Atomic absorption spectrometry and scanning electron microscopy evaluation of concentration of calcium ions and smear layer removal with root canal chelators. J Endod ,2009;35:727-30. Caligiani A, Acquotti D, Palla G, Bocchi V. Identification and quantification of the main organic components of vinegars by high resolution 1 H NMR spectroscopy. Analyt Chim Acta, 2007 ;585:110-9. 14- Carvalho-Junior J, Guimarães L, Correr-Sobrinho L, Pecora J, Sousa-Neto M Evaluation of solubility, disintegration, and dimensional alterations of a glass ionomer root canal sealer. Braz Dent J. ,2003, 114:118-14 15- Sousa-Neto M, Passarinho-Neto J, Carvalho-Junior J, Cruz-Filho A, Pecora J, Saquy P Evaluation of the effect of EDTA, EGTA and CDTA on dentin adhesiveness and microleakage with different root canal sealers. Braz Dent J, 2002, 123:128-13. 16- Tagger M, Tagger E, Tjan A, Bakland L Measurement of adhesion of endodontic sealers to dentin. J Endod , 2002, 351:354-28 17- Camps J, Pommel L, Bukiet F, About I. Influence of the powder/liquid ratio on the properties of zinc oxide-eugenol-based root canal sealers. Dent Mater. ,2004;20:915–23. 18- Takatsuka T, Tanaka K, Iijima Y. Inhibition of dentine demineralization by zinc oxide: In vitro and in situ studies. Dent Mater. ,2005;21:1170–7. 19- Wong RH, Palamara JE, Wilson PR, Reynolds EC, Burrow MF. Effect of CPP-ACP addition on physical properties of zinc oxide non-eugenol temporary cements. Dent Mater. ,2011;27 : 329–38 20- Ahlberg K, Tay W. A methacrylate-based cement used as a root canal sealer. Int Endo J. ,1998;31: 15-5. 21- Grossman L. Physical properties of root canal cements. J Endod, 1976;2: 166. 22- Limkangwalmongkol S, Abbott P, Sandler A. Apical dye penetration with four root canal sealers and gutta-percha using longitudinal sectioning. J Endod. 1992;18: 535-16. 23- Leyhausen G, Heil J, Reifferscheid G, Waldmann P, Geurtsen W. Genotoxicity and cytotoxicity of the epoxy resin-based root canal sealer AH plus. J Endod. 1999;25: 109-8. 24- Leonardo M, Silva L, Almeida W, Utrilla L. Tissue response to an epoxy resin-based root canal sealer. Endod Dent Traumatol. 1999; 15:28-4. 25- Al-Khatar N, Kunzelmann K, Hickel R. Apical leakage of new root canal sealers (abstract). J Dent Res. 1995;74: 945-12. 26- De Almeida W, Leonardo M, Tanomaru Filho M, Silva L. Evaluation of apical sealing of three endodontic sealers. Int Endod J. 2000;33: 25-4. 27- Miletic I, Ribaric S, Karlovic Z, Silvana J, Bosnjac A, Anic I. Apical leakage of five root canal sealers after one year of storage. J Endod. 2002;28: 431-5. 28- Abramovitz I, Weinberg G, Borenstein A, Polak D, Kesler-Shvero D, et al. InVitro Biocompatibility of Endodontic Sealers Incorporating Antibacterial Nanoparticles. journal of nanomaterials. 2012;69:72-15. 29- Kennedy W, Walker W, Gouch R Smear layer removal effects on apical leakage. J Endod, 1986 ;12: 21–7. 30- Çalt S, Serper A. Time dependent effects of EDTA on dentin structures. J Endod 2002;28:17-9. 31- Grossman D, Louis l ,Dm D physical properties of root canal cements. J Endod 1976;166:175-6. 32- Qrastavik D, Eriksen H, Beyer-O. Adhesive properties and leakage of root canal sealer in vivo. Int Endod J 1983; 16:59-63. 33- Tagger M, Tagger E, Tjan A, Bakland L. shearing bond strength on endodontic sealers to gutta percha .J Endod 2003; 249:51-5. 34- Saleh I, Ruyter I, Haapasalo P , Orstavik D. Adhesion of endodontic sealers:scanning electron microscopy and energy dispersive spectroscopy . J Endod 2003;29:595-1. 35- Neelakantan P, Varughese A, Sharma S, Subbarao C, Zehnder M, De-Deus G. Continuous chelation irrigation improves the adhesion of epoxy resin-based root canal sealer to root dentine. Int Endod J. 2012 ;1097:102-12 36- Palaniswamy U, Kaushik M, Ram Surender L, Prashar N, Shikha Arya S, Srikanth Pasari S. A SEM evaluation of smear layer removal using two rotary instrument systems with EDTA and vinegar as a root canal irrigant. Journal of Restorative Dentistry 2016;41:17-21. 37- Candeiro G, de Matosa I, da Costa C, Fonteles C, do Vale M .A comparative scanning electron microscopy evaluation of smear layer removal with apple vinegar and NaOCL associated with EDTA. J Appl Oral Sci 2011; 1678-7757-4 38- Baumgartner JC, Mader CL. A scanning electron microscopic evaluation of four root canal irrigation regimens. J Endod ,1987; 147:57-13 39- Leonardo M, Silva L, Almeida W, Utrilla L.Tissue response to an epoxy resin-based root canal sealer. Endodont Dent Traumatol. 1999;15: 28-44 | ||||
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