Validated HPLC Method for Simultaneous Determination of Aripiprazole and Co-Administered Clonazepam in Spiked Human Plasma | ||||
| Journal of Pharmaceutical and Applied Chemistry | ||||
| Volume 3, Issue 1, January 2017, Page 5-9 PDF (678.55 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.18576/jpac/030108 | ||||
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| Authors | ||||
| Christine M. El-Maraghy1; Hesham Salem2; Sawsan M. Amer3; Marianne Nebsen4 | ||||
| 1Analytical Chemistry department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 11787 6th October city, Egypt. | ||||
| 2Pharmaceutical Analytical Chemistry department, Faculty of Pharmacy, Deraya University, Minia, Egypt. | ||||
| 3Analytical Chemistry department, Faculty of Pharmacy, Cairo University, Kasr-El Aini Street, 11562Cairo, Egypt. | ||||
| 43Analytical Chemistry department, Faculty of Pharmacy, Cairo University, Kasr-El Aini Street, 11562Cairo, Egypt. 4Pharmaceutical Analytical Chemistry department, Faculty of Pharmacy & Drug Technology, Heliopolis University, 3 Cairo Belbeis desert road, 2834El- Horria, Cairo, Egypt. | ||||
| Abstract | ||||
| A sensitive and selective high performance liquid chromatographic method was developed and validated for the simultaneous quantification of Aripiprazole and co-administered Clonazepam in spiked human plasma. Aripiprazole, Clonazepam and the internal standard Citalopram were extracted from plasma and analyzed on a reversed-phase C18 column using a mobile phase consisting of acetonitrile and 0.05M potassium dihydrogen orthophosphate (pH= 3.6) in a ratio of (40: 60, v/v). The ultraviolet (UV) detection was at 220 nm. The method was linear over concentration range (20-1000 ng mL-1) for both drugs. The method is very simple and allows obtaining a very good recovery of the analytes. | ||||
| Keywords | ||||
| Aripiprazole; Clonazepam; plasma; HPLC; co-administered | ||||
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