ATRAZINE DEGRADATION BY Bacillus megaterium ISOLATED FROM AGRICULTURAL SOIL | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 4, Volume 33, Issue 10, October 2008, Page 7477-7485 PDF (592.87 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2008.200726 | ||||
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| Authors | ||||
| M.M.A. El-Sawah1; Samia M.M. Bauoymy1; Eman H. Ashour1; Lobna A. Moussa2; Samah A. H. Shady1 | ||||
| 1Microbiol. Dept., Fac. Agric., Mansoura Univ., Mansoura, Egypt. | ||||
| 2Soil, Water and Environ. Res. Instit., Agric. Res. Center (ARC), Giza. | ||||
| Abstract | ||||
| In present study the capability of some microorganisms isolated from Egyptian soil to a triazine herbicide, atriazine, decomposition was assessed. Nine atrazine-degrading bacteria were isolated from soil that received repeated exposures of the commonly used herbicides atrazine. These isolates were belonged to genera Basilllus, Pseudomonas and Micrococcus. One isolate showed good growth and clearing zone on mineral salts agar media amended with atrazine (at 500 ppm) as a carbon and/or nitrogen source. This most active strain involved in atrazine degradation were selected and identified. The strain was classified as Bacillus megaterium. It degraded 45.8% of initial concentration of atrazine concurrent with increasing the population size from 105 to 108 CFU/ml culture. Atrazine-degrading enzymes by B. megaterium appear to be induced. No released chloride was detected from B. megaterium culture indicating that the triazine ring may be remained intact and the atrazine-metabolites not hydroxyatrazine. Atrazine metabolites may be deethylatrazine or deisopropylatrazine. These results indicate that B. megaterium can play an important role in the bioremediation of atrazine-contaminated sites. | ||||
| Keywords | ||||
| atrazine; Basilllus; Pseudomonas and Micrococcus; degradation; Bacillus megaterium; bioremediation | ||||
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