Chlorfenapyr Induce Oxidative Phosphorylation Deficiency in Exposed Rat and the Quinoa Effective Role
Abdel-Razik, R., Hamed, N. (2021). Chlorfenapyr Induce Oxidative Phosphorylation Deficiency in Exposed Rat and the Quinoa Effective Role. EKB Journal Management System, 42(4), 809-822. doi: 10.21608/asejaiqjsae.2021.201175
Reda Khamis Abdel-Razik; Nadia Ali Hamed. "Chlorfenapyr Induce Oxidative Phosphorylation Deficiency in Exposed Rat and the Quinoa Effective Role". EKB Journal Management System, 42, 4, 2021, 809-822. doi: 10.21608/asejaiqjsae.2021.201175
Abdel-Razik, R., Hamed, N. (2021). 'Chlorfenapyr Induce Oxidative Phosphorylation Deficiency in Exposed Rat and the Quinoa Effective Role', EKB Journal Management System, 42(4), pp. 809-822. doi: 10.21608/asejaiqjsae.2021.201175
Abdel-Razik, R., Hamed, N. Chlorfenapyr Induce Oxidative Phosphorylation Deficiency in Exposed Rat and the Quinoa Effective Role. EKB Journal Management System, 2021; 42(4): 809-822. doi: 10.21608/asejaiqjsae.2021.201175

Chlorfenapyr Induce Oxidative Phosphorylation Deficiency in Exposed Rat and the Quinoa Effective Role

Alexandria Science Exchange Journal
Article 3, Volume 42, Issue 4, October 2021, Page 809-822  XML PDF (365.26 K)
Document Type: Original Article
DOI: 10.21608/asejaiqjsae.2021.201175
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Authors
Reda Khamis Abdel-Razik email orcid 1; Nadia Ali Hamed email 2
1Pesticides Toxicity to Mammals and Aquatic Organisms, Central Agricultural Pesticide Lab., Agricultural Research Center, Alexandria, Egypt
2Researcher Pesticides Toxicity to Mammals and Aquatic Organisms, Central Agricultural Pesticide Lab., Agricultural Research Center, Alexandria, Egypt
Abstract
The present study investigates uncouple and pro-oxidant effects of chlorfenapyr (CFp) on rat liver and spleen mitochondria respiratory complexes and evaluates the quinoa seeds (Q) (30% from the basal diet) effects on CFp exposed rats. Repeated sublethal oral doses of CFp (180 mg / Kg body weight; 1/3 LD50) was given to rat. Liver and spleen mitochondria were isolated at the end of the fourth week after dosing. Changes in liver and spleen mitochondrial respiration complexes, and concomitant oxidative stress and antioxidant alterations were examined.  Since, CFp provokes a significant reduction in the activity of NADH dehydrogenase (complex I), succinate dehydrogenase (complex II), ubiquinone cytochrome c oxidoreductase (complex III), cytochrome c oxidase (complex IV), superoxide dismutase (SOD), and reduced glutathione (GSH) level. In addition, a significant increase in ATP synthase (complex V) and malondialdehyde (MDA) levels were observed in both liver and spleen mitochondria, indicating clearly an intense peroxidation within mitochondria. So, the results conclude that hepatic and splenic respiratory complexes in rats were severely compromised following CFp exposure. Moreover, Q enhanced diet neutralizes the CFp adverse effect that encourages its use as a mitochondrial enriches source and a protective antioxidant food supplement.
 
Keywords
Chlorfenapyr; Electron transport chain; Mitochondria; Oxidative stress; Quinoa
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