Evaluation of the Potential Therapeutic Anti-inflammatory, Anti-proliferative and Apoptotic Effects of Viburnum tinus Leaves Different Extracts. | ||||
| Egyptian Journal of Chemistry | ||||
| Article 10, Volume 65, Issue 4, April 2022, Page 95-110 PDF (1.08 MB) | ||||
| Document Type: Review Articles | ||||
| DOI: 10.21608/ejchem.2021.97025.4540 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Fatma Hassan Salman   1; Ekrami Ahmed Hassan2; Mona Abdel-Motagaly3; soad ali sherif4; Mona Nagiub Moharib5; Eman M. Abd ElAzeem 6
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| 1Biochemistry and molecular biology department,theodor bilharz research institute,giza,egypt | ||||
| 2biochemistry department, faculty of science , ain shams university | ||||
| 3Theodor Bilharz Research Institute | ||||
| 4biochemistry department, theodor bilharz research institute | ||||
| 5Biochemistry department ,theodor bilharz research institute | ||||
| 6Biochemistry Dept., Faculty of Science, Ain Shams University,Abbassia,Cairo, Egypt. | ||||
| Abstract | ||||
| Viburnum tinus (V.tinus) is an Adoxaceae family medicinal plant rich in vital phytochemicals such as polyphenols, triterpenoids, flavonoids, coumarins, and iridoids. This study shows that V. tinus leaf extracts have anti-inflammatory, anti-cancer, and apoptotic properties. The plant leaves were dried and soaked in 90% MeOH to prepare the crude extract, which was fractionated by n-Hexane, CH2Cl2, and MeOH. Phytochemicals were detected in these extracts, and their anti-inflammatory, anti-cancer, and apoptotic activities were evaluated in-vitro.The highest content of flavonoids, tannins, terpenoids, and coumarins in the n-Hexane extract stimulates its anti-inflammatory activity, as evidenced by protein denaturation inhibition (94.60±3.90 percent), proteinase inhibition (88.5±0.50 %), membrane stabilization (73.2±1.58 %), and inflammatory cytokine (INF- ɤ) inhibition (100±0.00 %) without any toxicity on blood. It also has a cytotoxic impact on HeG2, MCF7, and CaCO2 cell lines, with IC50 (29.7 2.58, 75.3 4.70, 29.1 0.59 µg/ml) and SI (10.2, 10.4, 4) on HeG2, MCF7, and CaCO2, respectively. The apoptotic impact of n-Hexane in HepG2 resulted in quantitative expression of the BAX gene, which increased significantly and the BCL2 gene, which decreased significantly. | ||||
| Keywords | ||||
| Viburnum tinus; anti-inflammatory; anti-cancer; apoptosis; DNA fragmentation; INF-gamma; pro and anti-apoptotic | ||||
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