miRNA-223-3p, miRNA- 2909 and Cytokines Expression in COVID-19 Patients Treated with Ivermectin | ||||
| Zagazig University Medical Journal | ||||
| Article 24, Volume 28, Issue 3, May 2022, Page 573-582 PDF (351.68 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/zumj.2021.92746.2329 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Noha Rezk  1; Ashraf Elsayed Sileem 2; Doaa Gad3; amr osam Khalil4
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| 1Medical biochemistry .zagazig university | ||||
| 2chest department, faculty of medicine, zagazig university | ||||
| 3Chest department, faculty of medicine, Zagazig University | ||||
| 4Lecturer of Radio-diagnosis, Faculty of Medicine – Zagazig University | ||||
| Abstract | ||||
| The role of Ivermectin in improving the outcome of coronavirus disease of 2019 (COVID-19) symptoms was reported in several studies, while its effect on the pro-inflammatory cytokines triggering the cytokine storm is still not investigated. This study aimed to investigate the role of Ivermectin on the proinflammatory cytokines in Covid-19 patients and correlated the results with the expression of miR-2909, miR-223-3p. Three hundred and twenty hospitalized patients with confirmed moderate-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection were selected. The patients were divided into 2 groups: Group I treated with the Egyptian protocol of COVID-19 including (Ivermectin plus hydroxychloroquine). Group II was treated with the Egyptian protocol, including hydroxychloroquine and no Ivermectin. IL-6, IL-1b, procalcitonin, and gene expression of miR-2909, miR-223-3p, and Toll-like receptor 4 were done by real-time Polymerase Chain Reaction (PCR). Patients treated with COVID-19 protocol including Ivermectin showed a significant decrease of cytokines levels (IL-6, IL-1, and procalcitonin), when compared with the other group, the cytokines levels improvement were positively correlated with miR-2029 expression and negatively correlated with the expression of miR-223-3p. Moderate ill COVID-19 patients treated with Ivermectin showed a significant decline in mortality rate and duration of hospital stay. Ivermectin is an effective drug in improving the outcome of SARS-CoV-2 patients with a significant decrease in mortality rate through decreasing cytokines expression via controlling miR-2029, miR-233-3p expressions. | ||||
| Keywords | ||||
| Interleukin-6; ivermectin; mrna | ||||
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