The value of Real time PCR and High Resolution Ultrasound in Diagnosis of Suspected Pure Neural Leprosy
Elbadawy, N. (2021). The value of Real time PCR and High Resolution Ultrasound in Diagnosis of Suspected Pure Neural Leprosy. EKB Journal Management System, 30(1), 45-52.
Nissreen E. Elbadawy. "The value of Real time PCR and High Resolution Ultrasound in Diagnosis of Suspected Pure Neural Leprosy". EKB Journal Management System, 30, 1, 2021, 45-52.
Elbadawy, N. (2021). 'The value of Real time PCR and High Resolution Ultrasound in Diagnosis of Suspected Pure Neural Leprosy', EKB Journal Management System, 30(1), pp. 45-52.
Elbadawy, N. The value of Real time PCR and High Resolution Ultrasound in Diagnosis of Suspected Pure Neural Leprosy. EKB Journal Management System, 2021; 30(1): 45-52.

The value of Real time PCR and High Resolution Ultrasound in Diagnosis of Suspected Pure Neural Leprosy

Egyptian Journal of Medical Microbiology
Article 6, Volume 30, Issue 1, January 2021, Page 45-52  XML PDF (498.72 K)
Document Type: New and original researches in the field of Microbiology.
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Author
Nissreen E. Elbadawy email
Assistant Professor of Medical Microbiology & Immunology, Faculty of Medicine, Zagazig University
Abstract
Background: Cases with pure neural leprosy (PNL) are difficult in their diagnosis and usually loss the early management opportunity. Objective: to evaluate Real time PCR and high resolution ultrasonography of peripheral nerves as diagnostic tools in PNL. Methodology The study included 25 suspected PNL and 25 healthy controls. All patients were subjected to ultrasonography cross-sectional area (CSA) measurement. Fine needle aspiration (FNA) was obtained, and followed by Ziehl-Neelsen (ZN)staining, and real time PCR. Results: ZN staining demonstrated M leprae bacilli in only 8 cases while their DNA was detected in 92 % cases (Sensitivity of both were 100%, and 44.4%, respectively). Only the left median and right and left posterior tibial nerves CSA measurements showed good performance in distinguishing patients of PNL.  Median nerve cut off=15.5 mm2, with a sensitivity of 72%), and right and left posterior tibial nerves CSA Cut Off were 12, 11.5 mm2 with sensitivity of 76-84%. Conclusions: ultrasonography of suspected nerves and real time PCR of nerve aspirates are simple accurate tests for diagnosis of PNL.
Keywords
Pure neural leprosy; real time; PCR; High resolution ultrasound
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