Metabolic Profiling of Endophytic Bacillus subtilis US2 Isolated from Rosmarinus officinalis Leaves with Potential Antimicrobial Activity | ||||
| Journal of Pharmaceutical and Applied Chemistry | ||||
| Volume 6, Issue 1, January 2020, Page 26-37 PDF (572 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.18576/jpac/060102 | ||||
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| Authors | ||||
| Magdy A. Abu-Gharbia; Rehab M. Mohamed; Mohamed A. Awad | ||||
| Botany and Microbiology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt. | ||||
| Abstract | ||||
| Twelve isolates of endophytic bacteria from Rosmarinus officinalis (Rosemary) have been screened for their potential in preventing growth of pathogens (Bacillus subtilis, Staphylococcus epidermidis, Serratia marcescens, Proteus vulgaris, Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, Fusarium oxysporum, Acremonium solani, Aspergillus flavus and Penicillium griseofulvum) in addition to non-pathogenic Aspergillus columnaris and Aspergillus ochraceus. Endophytic bacterial isolates displayed considerable antimicrobial activity. The mean zones of inhibition produced by the different bacterial endophytes fluctuated between one and 49 mm. The most active endophyte inhibited growth of Staphylococcus epidermidis (28.67mm), Serratia marcescens (48.67mm) and Bacillus cereus (10mm). 16S rRNA sequence has been deposited in the NCBI GenBank database under accession number: MH093646 (Bacillus subtilis). Metabolic profiling analysis by GC-MS revealed that five compounds were major constituents of the crude extract obtained from endophytic Bacillus subtilis MH093646; (Cyclo-hexanone / 2-butoxy-Ethanol / Acetic acid, butyl ester / Propanoic acid, ethyl ester and 2-Butoxyethyl acetate). Twelve chemical constituents were identified from the crude extract of Rosmarinus officinalis. Alpha-Pinene / Camphene / 3-Carene / p-Cymene / D-Limonene / Bornyl acetate and Caryophyllene compounds had antimicrobial properties. Data indicated that antimicrobial activity of Rosmarinus officinalis is not related to the activity of the endophytic bacteria. | ||||
| Keywords | ||||
| Endophyte; Rosmarinus officinalis; Bacillus subtilis; 16s rRNA; Antimicrobial activity and GC-MS | ||||
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