Biocontrol of Acinetobacter baumannii-S-MH Using Bacteriophages Isolated from Sewage Water
O., A., Esmael, A., Abdelrahman, S., Shraf, A., Hassan, M. (2021). Biocontrol of Acinetobacter baumannii-S-MH Using Bacteriophages Isolated from Sewage Water. EKB Journal Management System, 13(2), 17-32. doi: 10.21608/eajbsg.2021.203035
Abdel-Monem M. O.; Ahmed Esmael; Soheir Abdelrahman; Amira Shraf; Mervat G. Hassan. "Biocontrol of Acinetobacter baumannii-S-MH Using Bacteriophages Isolated from Sewage Water". EKB Journal Management System, 13, 2, 2021, 17-32. doi: 10.21608/eajbsg.2021.203035
O., A., Esmael, A., Abdelrahman, S., Shraf, A., Hassan, M. (2021). 'Biocontrol of Acinetobacter baumannii-S-MH Using Bacteriophages Isolated from Sewage Water', EKB Journal Management System, 13(2), pp. 17-32. doi: 10.21608/eajbsg.2021.203035
O., A., Esmael, A., Abdelrahman, S., Shraf, A., Hassan, M. Biocontrol of Acinetobacter baumannii-S-MH Using Bacteriophages Isolated from Sewage Water. EKB Journal Management System, 2021; 13(2): 17-32. doi: 10.21608/eajbsg.2021.203035

Biocontrol of Acinetobacter baumannii-S-MH Using Bacteriophages Isolated from Sewage Water

Egyptian Academic Journal of Biological Sciences, G. Microbiology
Article 3, Volume 13, Issue 2, December 2021, Page 17-32  XML PDF (1.1 MB)
Document Type: Original Article
DOI: 10.21608/eajbsg.2021.203035
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Authors
Abdel-Monem M. O.1; Ahmed Esmael1; Soheir Abdelrahman2; Amira Shraf1; Mervat G. Hassan1
1Botany and Microbiology Department, Faculty of Science, Benha University, Benha 13511, Egypt.
2Clinical pathology Department, Faculty of Medicine, Benha University, Benha 13511, Egypt.
Abstract
The aim of this study is to isolate and characterize bacteriophages, as an alternative solution against multidrug-resistant Acinetobacter baumannii-S-MHbacteria causing an outbreak in hospital environments and intensive care units (ICUs). Here, biochemically; isolating and characterizing Acinetobacter baumannii-S-MHby the automated Vitek 2 system. Isolated Acinetobacter baumannii -S-MHbacteria was tested against a collection of Antibiotics by Antibiotic sensitivity test. Acinetobacter baumannii showed resistance to most of the antibiotics tested. To overcome this problem, Acinetobacter baumannii's phage (VB_AciM-AM-M) was isolated, identified, and applied to control the growth of this opportunistic pathogen. Morphologically, phage was identified by TEM; showed that (VB_AciM-AM-M) phage is shaped Myoviridae morphotype. The one-step growth curve of the phage showed burst sizes of 290 PFU/cell with a latent period of 20 minutes for (VB_AciM-AM-M) phage. Complete inhibition of bacterial growth was achieved using phage with MOIs of 1, 3 and 5 after 1, 3, and 24 h. of incubation at 37°C. Hence, this study indicates that the isolated bacteriophages are promising biocontrol agents that could challenge antibiotic-resistant Acinetobacter baumannii-S-MHbacteria to announce new successful alternatives to antibiotics.
Keywords
Acinetobacter baumannii -S-MH; Antibiotics; Bacteriophages and Biocontrol
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