ANTIBACTERIAL ACTIVITIES OF SOME ISOLATED ALGAE FROM SIWA OASIS-EGYPT
Wafaa, S., A., Afaf, H., F., Hanona, S., Y., M. A., D., Yosra, E., M. (2017). ANTIBACTERIAL ACTIVITIES OF SOME ISOLATED ALGAE FROM SIWA OASIS-EGYPT. EKB Journal Management System, 39(3), 1-23. doi: 10.21608/jes.2017.20361
Abou El-Kheir Wafaa, S.; Farag, Afaf, H.; Yaakob, Hanona, S.; Darwish, M. A.; Moustafa, Yosra, E.. "ANTIBACTERIAL ACTIVITIES OF SOME ISOLATED ALGAE FROM SIWA OASIS-EGYPT". EKB Journal Management System, 39, 3, 2017, 1-23. doi: 10.21608/jes.2017.20361
Wafaa, S., A., Afaf, H., F., Hanona, S., Y., M. A., D., Yosra, E., M. (2017). 'ANTIBACTERIAL ACTIVITIES OF SOME ISOLATED ALGAE FROM SIWA OASIS-EGYPT', EKB Journal Management System, 39(3), pp. 1-23. doi: 10.21608/jes.2017.20361
Wafaa, S., A., Afaf, H., F., Hanona, S., Y., M. A., D., Yosra, E., M. ANTIBACTERIAL ACTIVITIES OF SOME ISOLATED ALGAE FROM SIWA OASIS-EGYPT. EKB Journal Management System, 2017; 39(3): 1-23. doi: 10.21608/jes.2017.20361

ANTIBACTERIAL ACTIVITIES OF SOME ISOLATED ALGAE FROM SIWA OASIS-EGYPT

Journal of Environmental Science
Article 1, Volume 39, Issue 3, September 2017, Page 1-23  XML PDF (630 K)
Document Type: Review Article
DOI: 10.21608/jes.2017.20361
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Authors
Abou El-Kheir Wafaa, S.1; Farag, Afaf, H.1; Yaakob, Hanona, S.2; Darwish, M. A.3; Moustafa, Yosra, E.2
1Botany Department, Faculty of Women for Arts, Science and Education, Ain Shams University
2Medicinal and Aromatic Plants Department, Desert Research Centre
3Fertilization Technology Department, National Research Centre
Abstract
In compliance to the recent surveys on algal species and their potentials to produce biologically active natural products, collection of algae were carried out during summer 2014 from five water habitats in Siwa Oasis. Three common microalgal pure isolates including one green alga Chlorella vulgaris and twoblue green algae Chroococcus turgidus and Phormidium tenue were obtained using BG11, Basal Bold and Zarrouk media. The methanolic extracts of the three algal species were tested for the antibacterial activity against eight human pathogenic bacteria Enterococcus sp., Staphylococcus aureus, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumonia, Proteus sp., Pseudomonas aeruginosa and Salmonella tyhphi using the agar well diffusion method. The results revealed that Phormidium tenue showed the highest promising antibacterial activity so it was choosed for successive extraction by using petroleum ether, ethylacetate, 80%methanol and water solvents. Among the tested solvents the ethyl acetate successive extract showed the highest antibacterial activity. Results of some chromatographic investigations for the ethyl acetate extract indicated that the phenolic compounds were the main components. High performance liquid chromatography (HPLC) technique was used for qualitative and quantitative analysis of the bioactive phenolic compounds. It resulted in identification of 22 phenolic compounds with different concentrations ranging from 1755.2 to10.425 μgg-1for ethyl vanillate and catechol compounds, respectively.
Keywords
Key words: Algae isolation; algal extract; Antibacterial activity; human pathogenic bacteria; High performance liquid chromatography (HPLC)
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