Screening and optimization of extracellular cellulase and pectinase enzymes produced from post-harvest fungi of apple (Pyrus malus L.) and tomato (Solanum lycopersicum L.)
El-said, A., A., H., Maghraby, T., Meghezel, S. (2017). Screening and optimization of extracellular cellulase and pectinase enzymes produced from post-harvest fungi of apple (Pyrus malus L.) and tomato (Solanum lycopersicum L.). EKB Journal Management System, 16(1), 21-36. doi: 10.21608/jesj.2017.204048
A. H. El-said; Hussein M. A.; Thanaa A. Maghraby; Safa M. Meghezel. "Screening and optimization of extracellular cellulase and pectinase enzymes produced from post-harvest fungi of apple (Pyrus malus L.) and tomato (Solanum lycopersicum L.)". EKB Journal Management System, 16, 1, 2017, 21-36. doi: 10.21608/jesj.2017.204048
El-said, A., A., H., Maghraby, T., Meghezel, S. (2017). 'Screening and optimization of extracellular cellulase and pectinase enzymes produced from post-harvest fungi of apple (Pyrus malus L.) and tomato (Solanum lycopersicum L.)', EKB Journal Management System, 16(1), pp. 21-36. doi: 10.21608/jesj.2017.204048
El-said, A., A., H., Maghraby, T., Meghezel, S. Screening and optimization of extracellular cellulase and pectinase enzymes produced from post-harvest fungi of apple (Pyrus malus L.) and tomato (Solanum lycopersicum L.). EKB Journal Management System, 2017; 16(1): 21-36. doi: 10.21608/jesj.2017.204048

Screening and optimization of extracellular cellulase and pectinase enzymes produced from post-harvest fungi of apple (Pyrus malus L.) and tomato (Solanum lycopersicum L.)

Journal of Environmental Studies
Article 3, Volume 16, Issue 1, March 2017, Page 21-36  XML PDF (472.99 K)
Document Type: High quality original papers
DOI: 10.21608/jesj.2017.204048
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Authors
A. H. El-said1; Hussein M. A.1, 2; Thanaa A. Maghraby2; Safa M. Meghezel email 3
1Biology Department, Faculty of Science, Taif University, Kingdom of Saudi Arabia.
2Botany Department, Faculty of Science, South Valley University, 83523 Qena, Egypt
3Botany and Microbiology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt.
Abstract
Fourty-eight species and two species varieties belonging to 19 genera were collected from 50 samples of apple and tomato fruits (25 from each) from Sohag governorate in Egypt on dichloran rose-Benegal chloramphenicol agar medium at 28ᴼC. The most common genera were Alternaria, Aspergillus, Cladosporium, Fusarium and Penicillium. Form the above genera the most prevalent species were Alternaria alternata, A. tenuissima, Aspergillus niger, A. flavus, A. fumigatus, Cladosporium cladosporioides, C. herbarum and Penicillium oxalicum. Fourty-eight species and two species varieties fungal species screened for their abilities to produce cellulase (C1 enzyme) and pectinase enzymes. Six and seven species showed high cellulolytic and pectinolytic activity, while 23 and 17 species were moderately activity for the two enzymes, respectively. The remaining species were low activity in both enzymes. The highest cellulase and pectinase activities were recorded by Aspergillus chevalieri. Maximum production of cellulase enzyme by A.chevalieri was obtained after 6 days of incubation at 30ᴼC with initial pH 6 in culture medium containing sucrose and peptone as carbon and nitrogen sources, respectively. Regarding to pectinase enzyme the highest pectinase production by A. chevalieri was recorded after 6 days of incubation at 30ᴼC with initial pH 8 in culture medium containing pectin and ammonium sulphate as carbon and nitrogen sources, respectively.
Keywords
Mycology; Postharvest diseases; Extracellular enzymes; Cellulase; Pectinase; Apple; Tomato; Aspergillus chevalieri
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