Biological control of Culex pipiens mosquito by local bacterial isolates
Kassem, Y., El-Baghdady, K., Saleh, N., Wahba, M. (2018). Biological control of Culex pipiens mosquito by local bacterial isolates. EKB Journal Management System, 14(2), 21-40. doi: 10.21608/ajbs.2018.205545
Yasmine E. Kassem; Khaled Z. El-Baghdady; Nagwa E.M. Saleh; Mahmoud M.I. Wahba. "Biological control of Culex pipiens mosquito by local bacterial isolates". EKB Journal Management System, 14, 2, 2018, 21-40. doi: 10.21608/ajbs.2018.205545
Kassem, Y., El-Baghdady, K., Saleh, N., Wahba, M. (2018). 'Biological control of Culex pipiens mosquito by local bacterial isolates', EKB Journal Management System, 14(2), pp. 21-40. doi: 10.21608/ajbs.2018.205545
Kassem, Y., El-Baghdady, K., Saleh, N., Wahba, M. Biological control of Culex pipiens mosquito by local bacterial isolates. EKB Journal Management System, 2018; 14(2): 21-40. doi: 10.21608/ajbs.2018.205545

Biological control of Culex pipiens mosquito by local bacterial isolates

African Journal of Biological Sciences
Article 2, Volume 14, Issue 2, 2018, Page 21-40  XML PDF (766.22 K)
Document Type: Original Article
DOI: 10.21608/ajbs.2018.205545
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Authors
Yasmine E. Kassem1; Khaled Z. El-Baghdady email orcid 2; Nagwa E.M. Saleh3; Mahmoud M.I. Wahba4
1Department of Microbiology, Faculty of Science, Ain Shams University
2Department of Microbiology, Faculty Science, Ain Shams Univ., Cairo, Egypt
3Research Studies and Training Center on Vectors of Diseases, Faculty of Science, Ain Shams University.
4Department of Entomology, Faculty of Science, Ain Shams University
Abstract
In this work, 62 bacterial and actinobacterial isolates were isolated from different soil samples and screened for their larvicidal activities against the 3rd instar Culex pipiens larvae. Two potent isolates that showed high mortality rate were identified by16S rRNA gene sequencing as Bacillus subtilis (MH370499) and Streptomyces griseoruber (MH370498). Optimizing the environmental and nutritional conditions increased the mortality percentage of B. subtilis from 82.9% to 100% and S. griseoruber from 69% to be 100%
LC50 and LC90 of B. subtilis were 13.68% and 28.29%, while for S. griseoruber were 5.79% and 18.53% respectively. The best solvent for active substance from B. subtilis extraction was ethyl acetate, while for S. griseoruber was hexane. The GC-MS analysis of S. griseoruber detected the presence of antilarval compounds ambrosin and triacontane. In addition, the larvicidal surfactin produced by B. subtilis was detected by haemolysis.
 
Keywords
Control; Culex; Streptomyces; Bacillus; mosquito
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