Multi Drug Resistant Pseudomonas aeruginosa in a Health Care Setting in Alexandria
Abaza, A. (2010). Multi Drug Resistant Pseudomonas aeruginosa in a Health Care Setting in Alexandria. EKB Journal Management System, 40(2), 333-347. doi: 10.21608/jhiph.2010.20608
Amany Abaza. "Multi Drug Resistant Pseudomonas aeruginosa in a Health Care Setting in Alexandria". EKB Journal Management System, 40, 2, 2010, 333-347. doi: 10.21608/jhiph.2010.20608
Abaza, A. (2010). 'Multi Drug Resistant Pseudomonas aeruginosa in a Health Care Setting in Alexandria', EKB Journal Management System, 40(2), pp. 333-347. doi: 10.21608/jhiph.2010.20608
Abaza, A. Multi Drug Resistant Pseudomonas aeruginosa in a Health Care Setting in Alexandria. EKB Journal Management System, 2010; 40(2): 333-347. doi: 10.21608/jhiph.2010.20608

Multi Drug Resistant Pseudomonas aeruginosa in a Health Care Setting in Alexandria

Journal of High Institute of Public Health
Article 8, Volume 40, Issue 2, April 2010, Page 333-347  XML PDF (191.51 K)
Document Type: Original Article
DOI: 10.21608/jhiph.2010.20608
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Author
Amany Abaza*
Microbiology Department, Alexandria University Students' Hospital, Egypt
Abstract
Background: The emergence of Multi drug resistant Pseudomonas aeruginosa (MDRPA) among intensive care unit (ICU) patients is increasingly recognized as a public health threat worldwide. Objective: This work aimed to study the occurrence of MDRPA among critically ill patients in a health care setting in Alexandria. Methods: During a 12 months period, different clinical samples (sputum, endotracheal aspirates, blood, urine, and pus) obtained from ICU patients were tested for the isolation and identification of Pseudomonas aeruginosa (P. aeruginosa) strains; that were screened for their antimicrobial susceptibility patterns using single disc diffusion method. Identified MDRPA strains were further tested for their susceptibility to polymyxin E (colistin), polymyxin B, and tigecycline. Polymerase chain reaction (PCR) assay was performed to detect VIM and IMP MBL genes. Results: Of the 105 P. aeruginosa strains isolated from various clinical samples, 20 (19%) were found to be MDRPA, of which 16 (80%) were sensitive to each of colistin and polymyxin B, while only 5 (25%) strains were sensitive to tigecycline. PCR assay revealed that 9 (45%) strains possessed VIM MBL gene and none (0%) harbored IMP MBL gene. Conclusion: The occurrence of MDRPA strains among critically ill patients in this study was noticeable; with colistin and polymyxin B being effective upon the majority of identified MDRPA strains, and VIM MBL gene was found to be significantly harbored.
Keywords
Multidrug Resistance Pseudomonas aeruginosa; Metallo Beta Lactamases; Polymyxins; Colistin; Tigecycline; VIM gene, IMP gene; MBL gene
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