Assessment of β-carotenoids extracted from Dunaliella salina microalgae:Toxicological aspects
K. El-Baz, F., Aly, H., Salama, A., Ali, S., I. El Askary, H., M. Khalil, A. (2022). Assessment of β-carotenoids extracted from Dunaliella salina microalgae:Toxicological aspects. EKB Journal Management System, 65(6), 727-739. doi: 10.21608/ejchem.2021.98823.4597
Farouk K. El-Baz; Hanan Farouk Aly; Abeer Salama; Sami I Ali; Hesham I. El Askary; Asmaa M. Khalil. "Assessment of β-carotenoids extracted from Dunaliella salina microalgae:Toxicological aspects". EKB Journal Management System, 65, 6, 2022, 727-739. doi: 10.21608/ejchem.2021.98823.4597
K. El-Baz, F., Aly, H., Salama, A., Ali, S., I. El Askary, H., M. Khalil, A. (2022). 'Assessment of β-carotenoids extracted from Dunaliella salina microalgae:Toxicological aspects', EKB Journal Management System, 65(6), pp. 727-739. doi: 10.21608/ejchem.2021.98823.4597
K. El-Baz, F., Aly, H., Salama, A., Ali, S., I. El Askary, H., M. Khalil, A. Assessment of β-carotenoids extracted from Dunaliella salina microalgae:Toxicological aspects. EKB Journal Management System, 2022; 65(6): 727-739. doi: 10.21608/ejchem.2021.98823.4597

Assessment of β-carotenoids extracted from Dunaliella salina microalgae:Toxicological aspects

Egyptian Journal of Chemistry
Article 62, Volume 65, Issue 6, June 2022, Page 727-739  XML PDF (2.41 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2021.98823.4597
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Authors
Farouk K. El-Bazorcid 1; Hanan Farouk Aly email orcid 2; Abeer Salamaorcid 3; Sami I Ali4; Hesham I. El Askaryorcid 5; Asmaa M. Khalil6
1Plant Biochemistry Department, National Research Centre, El Buhouth St., Cairo, 12622, Egypt.
2Deprtment of Therapeutic Chemistry , National Research Centre (NRC),El Behouth St. , P.O.12622. Dokki, Giza, Egypt.
3pharmacology, NRC
4Associate prof. Plant Biochemistry Department ,NRC
5Department of Pharmacognosy ,Faculty of Pharmacy ,Cairo University ,Cairo
6Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo
Abstract
The acute and chronic toxicity of the β-carotenoid-rich fraction (BCRF) from Dunaliella salina (Chlorophyta) microalgae was evaluated by the supplementation of high doses of BCRF to experimental animals. The HPLC analysis showed that ‎hexane crude extract of D. salina contains 0.813%‎ of β-carotene. Various biomarkers were involved in this study, which includes hematological and biochemical analyses such as complete blood count, liver enzymes, and kidney function. In addition albumin and glucose levels were determined. The histopathological examination was also performed on hepatic, renal, and cardiac architectures to evaluate its safety. An acute toxicity study revealed no toxicological relevance post administration of serial doses (500 mg/kg body weight up to 5000 mg/kg b.wt.).The supplementation of experimental animals (male and female mice and rats) with a dose of 500 mg/kg body weight of βCRF of D. salina daily for three consecutive months did not show any toxicity symptoms in both genders of mice and rats and/or mortality. Histopathological observation exhibited no noticeable change in the liver, kidney and heart architectures of both male and female mice and rats. Therefore, BCRF of D. salina LD50 is up to 5000 mg/kg, and it is safe at the dose of 500 mg/kg b.wt. in chronic toxicity.
Keywords
Dunaliella salina; β carotene; Biochemcal Functions; hematological parameters; Histopathological investigation
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