Comparative Study of the Mesenchymal Stem Cell and Simvastatin in the Treatment of Hepatic Fibrosis in Rats Induced by Carbon Tetrachloride | ||||
| Benha Medical Journal | ||||
| Article 192, Volume 39, Special issue (Academic), September and October 2022, Page 78-95 PDF (1.61 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bmfj.2021.80486.1426 | ||||
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| Authors | ||||
Hanan Ibrahim Elkerdasy  1; Ali Mohamed Ali Mohamed2; Essam Mohammed Elsaid Eid3; marian victor zaki2; Naglaa Ali Saber Sarg4
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| 1anatomy department faculty of medicen benha university | ||||
| 2anatomy department, faculty of medicine, benha university | ||||
| 3Professor & Head of Anatomy & Embryology ,Department , faculty of medicine, Benha University | ||||
| 4Assistant Professor of Anatomy & Embryology, Faculty of medicine , Benha University | ||||
| Abstract | ||||
| Background: Hepatic fibrosis is an important medical disease with high rates of morbidity and mortality. Bone marrow mesenchymal stem cells have been recommended as a powerful therapy for treatment of liver fibrosis. Simvastatin has an ameliorative effect on fibrosis of different organs. The study aimed at comparing between the effect of BM-MSCs and simvastatin in treatment of liver fibrosisinduced by CCL4. Material and Methods: Fifty rats were categorized into, group I control group, group II in which rats injected by CCl4 to induce hepatic fibrosis. Group III in which rats were injected by CCL4,then injected intravenously by a single dose of BM-MSCs, group IV in which rats were injected by CCL4 then given simvastatin orally once daily for eight weeks. Group V in which rats were given both BM-MSC and simvastatin. At the end of the experimental study, blood samples were collected for biochemical analysis and liver tissues were prepared for histological and immunohistochemical examination. Results: CCL4 significantly elevated liver enzymes and induced destruction of normal hepatic structures with significant elevation of mean area percentage of collagen fibers deposition and TGF-b expression. BM-MSC and simvastatin improved liver enzymes and histological structure of liver tissue, but the anti-fibrotic effect of BM-MSC was superior to that of simvastatin. Moreover, combination of BM-MSC and simvastatin exerted strong anti-fibrotic effect and preserved normal histologic structure of liver tissue more than each of them alone. Conclusion: A combination of BM-MSCs and simvastatin has an ameliorative role in the treatment of liver fibrosis induced by CCl4. | ||||
| Keywords | ||||
| Liver fibrosis; CCL4; BM-MSCs; simvastatin; TGF b | ||||
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