Influence of Carbon Sources and Amino Acids on in vitro Propagation of Four Citrus Rootstocks | ||||
| Assiut Journal of Agricultural Sciences | ||||
| Article 10, Volume 47, Issue 5, October 2016, Page 151-164 PDF (373.72 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajas.2016.2068 | ||||
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| Abstract | ||||
| Shoot tip explants (0.5 cm) of four citrus rootstocks (trifoliate orange, sour orange, volkamariana and lime) were cultured on MS medium including vitamins supplemented with100 mg/l myo-inositol and 2.5 g/l gelrite. Three types of sugars (sucrose, fructose, glucose)were used at different concentrations (88, 132 and 176 mM) and two types of amino acids were used astryptophan at 0, 50, 75 and100 µM and glutathione at 0, 5, 10 and 20 µM. Growth regulators were used during multiplication and rooting stages as follow: a) multiplication,BAP at 1.0 mg/l and b) rooting, IBA at 0.5 mg/l. Sucrose was most effective on shoot and internode length and roots/shoot butthe number of shoots/explant, number of nodes and rooting percentage were more pronounced on the medium containing glucose, while fructose was more effective on the root length. Concerning amino acids, generally tryptophan was more effective on vegetative and rooting growth compared to glutathione. | ||||
| Keywords | ||||
| micropropagation; sucrose%; Fructose; glucose; tryptophan; glutathione; proliferation; Rooting; Poncirustrifoliata; Citrus aurantium; Citrus volkamariana; Citrus aurantifolia | ||||
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