Genotypic and Phenotypic Screening of Carbapenemase Mediated Resistance in Klebsiella pneumoniae Clinical Isolates: A Hospital Based Study. | ||||
| Al-Azhar University Journal of Virus Researches and Studies | ||||
| Article 5, Volume 3, Issue 1, 2021, Page 1-12 PDF (361.09 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/aujv.2021.207065 | ||||
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| Abstract | ||||
| Carbapenem-resistant Klebsiella pneumoniae (CRKP) has been reported increasingly all over the world and it is usually resistant to multiple antimicrobial agents. Is to detect the frequency and distribution of carbapenemase genes in CRKP at Al-Zahraa University hospital, Cairo, Egypt, and to compare between the performance of Rapidec Carba NP and modified carbapenemase inactivation (mCIM) phenotypic methods for early and reliable recovery of carbapenemase production in Klebsiella. pneumoniae (KP) clinical isolates. Sixty K. pneumoniae isolates were recovered in our Microbiology laboratory. Carbapenemase production by these isolates was assessed using antimicrobial sensitivity testing, Rapidec Carba NP, modified carbapenemase inactivation methods (mCIM) and detection of carbapenemase genes by PCR. CRKP was implicated in several types of healthcare associated infections with frequency of 46.7 (28/60). The frequency of carbapenemase genes was 13 (54.2) for blaNDM, 7 (29.2%) for blaOXA-48 and 1(4.1%) for blaKPC. 3 (12.5%) isolates carried both blaNDM and blaOXA-48. All isolates were negative for blaIMP, the sensitivity and specificity of Rapidec Carba NP test were (100%) and (91.7%) respectively, while sensitivity and specificity of mCIM were (66.7%) and (88.9 %) respectively, in comparison to the PCR test which is used as the gold standard test. The Carbapenem resistance is frequently encountered in K. pneumoniae isolates at our hospital. Rapidec carba NP and mCIM tests have high performance and applicability in detection of carbapenemases. However, The Rapidec carba NP was found to be rapid and with higher sensitivity. | ||||
| Keywords | ||||
| Klebsiella pneumoniae; Carbapenem-resistance; Rapidec Carba NP; Carbapenemes Inactivation Method; Carbapenemase genes | ||||
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