NATURAL INFECTION PEANUT WITH Peanut mottle virus (PEMV) IN EGYPT. | ||||
| Journal of Plant Production | ||||
| Article 10, Volume 32, Issue 4, April 2007, Page 2511-2525 PDF (689.25 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2007.207492 | ||||
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| Authors | ||||
| Eman A. Khattab; Manal A. EL-Shazly; Hayam S. Abdelkader | ||||
| Virus and Phytoplasma Res. Dept, Plant Path. Res. Instit., (ARC), Giza, 12619, Egypt. | ||||
| Abstract | ||||
| A virus causing mottling, yellowing, necrosis, malformation and stunting on Arachis hypogaea L. plants was identified as Peanut mottle virus (PeMV) on the basis of host range, symptomatology, serology and RT-PCR. The purified virus formed a single zone in the density gradient columns. The absorption spectrum of the purified virus had Amax at 260 nm and Amin at 245 nm. The A 260/280 and Amax/min ratios were 2.6 and 1.3, respectively. The estimated yield of the purified virus was 0.7 mg / 100g of peanut tissue. The virus particles had about 720-750 nm long and 13 nm width. Antiserum produced against PeMV had a titer of 1/1024 using indirect ELISA. Reverse transcription-polymerase chain reaction (RT-PCR) based method was developed for testing peanut (Arachis hypogaea L.) plants for infection by Peanut mottle virus (PeMV). The PCR product (340 bp) of PeMV/CP was successfully amplified by using one set of specific primers designed from conserved sequences within the capsid region of the virus. A good correlation was obtained between virus detection by RT-PCR method and virus detection from the same plants by enzyme-linked immunosorbent assay (ELISA). | ||||
| Keywords | ||||
| PeMV; electron microscopy; ELISA; TBIA; DBIA; and RT-PCR | ||||
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