USE OF PROTEIN ELECTROPHORESIS TO QUANTIFY RESISTANCE OF COTTON TO FUSARIUM WILT DISEASE | ||||
| Journal of Plant Production | ||||
| Article 10, Volume 32, Issue 5, May 2007, Page 3475-3488 PDF (867.92 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2007.208136 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| A. A. Aly; E. M. Hussein; M. R. Omar; A. M. A. El-Samawaty | ||||
| Plant Pathology Research Institute, Agric. Res. Center, Giza, Egypt. | ||||
| Abstract | ||||
| Six experimental crosses and 6 commercial cultivars of Egyptian cottons (Gossypium barbadense L.) were evaluated for Fusarium-wilt resistance under greenhouse conditions in 2007 growing season. The experimental crosses showed varying levels of susceptibility to Fusarium wilt disease, while the commercial cultivars were highly resistant. Seed proteins of the genotypes (crosses and cultivars) were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and the obtained banding patterns were visualized by using the Coomassie Brilliant Blue staining system. Data for Fusarium wilt ratings and amounts of protein fractions were entered into a computerized stepwise multiple regression. Using the predictors supplied by stepwise regression, a five-factor model was constructed to predict wilt incidence. This model showed that wilt incidence differences were due to the protein fractions nos. 4, 30, 32, 7 and 55, which accounted for 99.98% of the total variation in wilt incidence ratings. This result indicates that SDS-PAGE of seed proteins may provide a supplementary assay to greenhouse tests to distinguish quantitatively between Fusarium-wilt-resistant or susceptible genotypes. | ||||
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