Soil actinomycetes molecular characterization for secondary metabolites production
Ullah, S., Muhammad, Z., Jehan, S., Zia, S., Hussain, Z., Hussain, S., Shirazi, R., Ullah, A., Raziq, A., Gul, A., Ullah, H. (2022). Soil actinomycetes molecular characterization for secondary metabolites production. EKB Journal Management System, 5(1), 40-44. doi: 10.21608/jabps.2021.98256.1141
Shakir Ullah; Zubaida Sher Muhammad; Saqlain Jehan; Samavia Zia; Zakir Hussain; Syed Amjad Hussain; Ramiz Raza Shirazi; Asad Ullah; Abdur Raziq; Anbareen Gul; Hayat Ullah. "Soil actinomycetes molecular characterization for secondary metabolites production". EKB Journal Management System, 5, 1, 2022, 40-44. doi: 10.21608/jabps.2021.98256.1141
Ullah, S., Muhammad, Z., Jehan, S., Zia, S., Hussain, Z., Hussain, S., Shirazi, R., Ullah, A., Raziq, A., Gul, A., Ullah, H. (2022). 'Soil actinomycetes molecular characterization for secondary metabolites production', EKB Journal Management System, 5(1), pp. 40-44. doi: 10.21608/jabps.2021.98256.1141
Ullah, S., Muhammad, Z., Jehan, S., Zia, S., Hussain, Z., Hussain, S., Shirazi, R., Ullah, A., Raziq, A., Gul, A., Ullah, H. Soil actinomycetes molecular characterization for secondary metabolites production. EKB Journal Management System, 2022; 5(1): 40-44. doi: 10.21608/jabps.2021.98256.1141

Soil actinomycetes molecular characterization for secondary metabolites production

Journal of advanced Biomedical and Pharmaceutical Sciences
Article 4, Volume 5, Issue 1, January 2022, Page 40-44  XML PDF (414.01 K)
Document Type: Original Article
DOI: 10.21608/jabps.2021.98256.1141
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Authors
Shakir Ullah1; Zubaida Sher Muhammad2; Saqlain Jehan1; Samavia Zia1; Zakir Hussain3; Syed Amjad Hussain4; Ramiz Raza Shirazi3; Asad Ullah5; Abdur Raziqorcid 6; Anbareen Gul7; Hayat Ullah email orcid 8
1Institute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, Pakistan.
2Department Zoology, Sardar Bahadur Khan Women University Quetta Baluchistan
3Department of Chemistry, Government College University Faisalabad Pakistan
4Department of Chemistry, The university of Punjab Lahore Pakistan
5Department of Microbiology, Faculty of Life Sciences and Informatics, Balochistan University of Information Technology Engineering and Management Sciences, Quetta, Pakistan.
6Department of Bioinformatics & Biotechnology, Faculty of Life Science, Government College University Faisalabad, Pakistan.
7Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan
8Government College University Faisalabad
Abstract
Antibiotic resistance issue is really unavoidable, increased due to decrease in production of novel secondary metabolites production. Actinomycetes have the potential to produce biological active compounds used as an antibacterial, antiviral, antifungal, antiprotozoal, antihelminthic, anticholesterol, anticancer, immunosuppressant, pesticides and herbicides. Current research study was design to assess actinomycetes for production secondary metabolites. For this purpose different soil samples were collected and with help of dilution method, actinomycetes were grown on enrichment. Actinomycetes were morphologically analysed under microscope and molecularly through PCR and 16S-rRNA gene-amplification. After confirmation, actinomycetes were grown on LB and GMC media have potential to produce bioactive secondary metabolites. Current research study results that actinomycetes have potential to produce bioactive secondary metabolites. LB-media is the best suitable-medium for growth of actinomycetes and bioactive secondary-metabolites production. It is suggested that actinomycetes strains from various environment should be assessed for novel bioactive secondary metabolites.
Keywords
Actinomycetes; antibiotics; 16S rRNA gene; PCR; Streptomyces
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