Isolation and Identification of Bovine Viral Diarrhea Virus from Ovaries of Egyptian heifer and cow in 2019
aboezz, Z., elnagar, S., El-Mohamady, R., El-Habbaa, A., ElNahas, E. (2021). Isolation and Identification of Bovine Viral Diarrhea Virus from Ovaries of Egyptian heifer and cow in 2019. EKB Journal Management System, 41(1), 42-44. doi: 10.21608/bvmj.2021.79311.1428
zeinab R.A. aboezz; samia abd-elkarim elnagar; Rania El-Mohamady; Ayman Said El-Habbaa; Ehab ElNahas. "Isolation and Identification of Bovine Viral Diarrhea Virus from Ovaries of Egyptian heifer and cow in 2019". EKB Journal Management System, 41, 1, 2021, 42-44. doi: 10.21608/bvmj.2021.79311.1428
aboezz, Z., elnagar, S., El-Mohamady, R., El-Habbaa, A., ElNahas, E. (2021). 'Isolation and Identification of Bovine Viral Diarrhea Virus from Ovaries of Egyptian heifer and cow in 2019', EKB Journal Management System, 41(1), pp. 42-44. doi: 10.21608/bvmj.2021.79311.1428
aboezz, Z., elnagar, S., El-Mohamady, R., El-Habbaa, A., ElNahas, E. Isolation and Identification of Bovine Viral Diarrhea Virus from Ovaries of Egyptian heifer and cow in 2019. EKB Journal Management System, 2021; 41(1): 42-44. doi: 10.21608/bvmj.2021.79311.1428

Isolation and Identification of Bovine Viral Diarrhea Virus from Ovaries of Egyptian heifer and cow in 2019

Benha Veterinary Medical Journal
Article 9, Volume 41, Issue 1, October 2021, Page 42-44  XML PDF (364.1 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2021.79311.1428
View on SCiNiTO View on SCiNiTO
Authors
zeinab R.A. aboezz1; samia abd-elkarim elnagar email 2; Rania El-Mohamady3; Ayman Said El-Habbaaorcid 4; Ehab ElNahas5
1Virology department, faculty of vet medicine, Benha university.
2virology , faculty of veterinary , benha university , benha , egypt
3Viral Diseases Research Unit, Animal Reproduction Research Institute (ARRI), Agricultural Research Center (ARC), Giza, Egypt
4Virology department, faculty of veterinary medicine, Benha university, Moshtohor, Egypt
5Virology Department, Faculty of Veterinary Medicine, Benha University, Moshtohor 13736, Kalubia, Egypt
Abstract
Abstract
Bovine viral diarrhea virus (BVDV) infections continue to cause significant losses in cattle population. Better isolation of BVDV may influence in significant prevention and control of BVDV. This study was designed to isolate and identify the circulating BVDV in Egyptian cows and heifers during 2019. The isolated virus was identified and detected by immune florescent assay and immune peroxidase technique followed by RT- PCR that confirmed circulation of non cytopathic BVDV-1 in Egyptian cows and heifers. Further molecular analysis is recommended to determine the molecular epidemiology of BVD isolate for updating vaccine strain as a successful preventive strategy. It is obvious from the obtained result that immune peroxidase technique and immune florescent assay is highly sensitive and specific tool for detection of cytopathic and non cytopathic BVDV; More over RT-PCR is a highly sensitive tool for genotyping of BVDV. Further molecular analysis is recommended to confirm these finding and determine the molecular epidemiology of BVD-1 isolate and update the nature of the future vaccine for successful preventive strategies
Keywords
Noncytopathic BVDV; Semen; MDBK; RT-PCR, Genotype1
Statistics
Article View: 258
PDF Download: 243