Molecular detection and phylogenetic analysis of ORF103 and P32 genes of Capripoxviruses isolated from naturally infected cattle and sheep from Kaliobyia province in Egypt. | ||||
| Benha Veterinary Medical Journal | ||||
| Article 10, Volume 41, Issue 1, October 2021, Page 54-50 PDF (523.99 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2021.79378.1429 | ||||
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| Authors | ||||
Shimaa Abd-Elhafeiz  1; Ayman El-Habbaa 2; Fouad Saad El-mayet 3; Lamya Ateya4; Ehab EL-Nahas5
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| 1virology,animal heath research institute, Hurghada branh | ||||
| 2Virology department, faculty of veterinary medicine, Benha university, Moshtohor, Egypt | ||||
| 3Virology department- faculty of Veterinary medicine- Benha University | ||||
| 4Animal Health Research Institute (AHRI), Benha branch, Agriculture Research Center, Egypt | ||||
| 5Department of virology, faculty of veterinary medicine, | ||||
| Abstract | ||||
| Although each capripoxvirus members including lumpy skin disease virus (LSDV), sheeppox virus (SPPV) and goatpox virus (GTPV), predominantly affect their specific host species and cause generous annual financial losses, they are no longer absolutely host-specific. The aim of the current study is to isolate and genetically characterize the CaPV strains from clinically affected sheep and cattle in Kaliobyia province, Egypt during an outbreak in 2017-2018. A total fifty samples of skin lesions and nodules were obtained from clinically suspected field cases of sheep and cattle, respectively. They were prepared and isolated on chorioallantoic membrane (CAM) of embryonated chicken eggs. Typical pock lesions were seen in seventeen of the positive samples. PCR detection targeting ORF103 and P32 genes was used to identify the isolated samples. Two from each sheep and cattle samples as well as the sheep vaccinal strain used in Egypt were further sequenced, and phylogenetic analysis performed to validate the viruses. The phylogenetic analysis revealed that the field isolates from sheep were more closely related to the LSDV field isolates than to the sheeppox virus. Consequently, molecular techniques based on the ORF103 and P32 genes can be used to classify and distinguish capripoxviruses. This finding could shed light on the LSDV epidemiology in the Kaliobyia governorate. Cross-species infection by LSDV in sheep may have occurred in this outbreak. So, further research on the comparative study of ANK gene sequences, host range factors, of the isolate strains is required to confirm this suggested cross-species infection by LSDV in sheep. | ||||
| Keywords | ||||
| LSDV; chorioallantoic membrane; sheeppox virus, p32, ORF103 | ||||
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