IN VITRO SHORT-TERM GERMPLASM PRESERVATION OF Myrtus communis L
El-Shihy, O., Ghallab, A., Gad, M., Abdel Salam, R. (2007). IN VITRO SHORT-TERM GERMPLASM PRESERVATION OF Myrtus communis L. EKB Journal Management System, 32(6), 4451-4474. doi: 10.21608/jpp.2007.208776
O. M. El-Shihy; A. M. Ghallab; Mervat M. A. Gad; Ranya R. Abdel Salam. "IN VITRO SHORT-TERM GERMPLASM PRESERVATION OF Myrtus communis L". EKB Journal Management System, 32, 6, 2007, 4451-4474. doi: 10.21608/jpp.2007.208776
El-Shihy, O., Ghallab, A., Gad, M., Abdel Salam, R. (2007). 'IN VITRO SHORT-TERM GERMPLASM PRESERVATION OF Myrtus communis L', EKB Journal Management System, 32(6), pp. 4451-4474. doi: 10.21608/jpp.2007.208776
El-Shihy, O., Ghallab, A., Gad, M., Abdel Salam, R. IN VITRO SHORT-TERM GERMPLASM PRESERVATION OF Myrtus communis L. EKB Journal Management System, 2007; 32(6): 4451-4474. doi: 10.21608/jpp.2007.208776

IN VITRO SHORT-TERM GERMPLASM PRESERVATION OF Myrtus communis L

Journal of Plant Production
Article 14, Volume 32, Issue 6, June 2007, Page 4451-4474  XML PDF (877.11 K)
Document Type: Original Article
DOI: 10.21608/jpp.2007.208776
View on SCiNiTO View on SCiNiTO
Authors
O. M. El-Shihy1; A. M. Ghallab1; Mervat M. A. Gad2; Ranya R. Abdel Salam2
1Plant Physiology Section, Agric. Botany Dept., Fac. of Agric., Cairo Univ., Giza, Egypt
2Timber Trees Dept., Horticulture Research Institute, A. R. C., Giza, Egypt
Abstract
Shoot tips were obtained from Myrtus communis tissue cultured shootlets and in vitro preserved either by cryopreservation protocols (through vitrification, encapsulation-dehydration) or by cold preservation (4°C), as well as control storage at culture room conditions, however, nodal stem was involved in dehydration protocol in order to be cryopreserved. Preservation was adopted for 1, 2 and 3 months durations; yet, control treatment was prolonged for one year. Several cryoprotection trials were adopted, involving DMSO and/or PEG and glycerol, prior to vitrification protocol. Cryopreserved explant recovery was tested, as well as shootlets regeneration following preservation processes underwent several investigations. Shoot tips precultured on 0.18 M sucrose recorded higher recovered survival, shootlets number, length and leaves number than on a higher concentration and than on mannitol, either for 2 or 4 days, however, vitrification treatment induced no shootlets proliferation. Regenerated shootlets growth characters (survival %, shootlets number and dry weights) induced after preservation under growth chamber conditions (control) for one year, recorded the highest observed results. Similarly, chemical composition (chlorophyll a, total chlorophylls, carotenoids, total indoles, total free amino acids, total sugars and indoles/phenols ratio) as well as essential oil content and components were augmented due to preservation under control conditions; however, fresh weight, chlorophyll b, as well as total soluble phenols were low. RAPD analysis proved that preserved shootlets maintained their genetic stability through germplasm preservation methods.
Keywords
Myrtus communis; cryopreservation; dehydration; encapsulation-dehydration; vitrification; essential oil; RAPD
Statistics
Article View: 51
PDF Download: 169