Development of duplex real-time PCR assay based on C18L and DNA pol genes for rapid diagnosis of camel pox in dromedary camels. | ||||
| Benha Veterinary Medical Journal | ||||
| Article 16, Volume 41, Issue 1, October 2021, Page 75-77 PDF (286.98 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2021.78296.1423 | ||||
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| Authors | ||||
Amir A Shehata   1; Ehab M ElNahas2; Eman M. Abo Hatab3; Saad Sharawi Ali 4; Hanaa A. Ahmed5
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| 1Genome Research Unit, Animal Health Research Institute | ||||
| 2Virology Department, Faculty of veterinary medicine, Benha University | ||||
| 3Virology Department, Animal Health Research Institute. | ||||
| 4Department of virology , faculty of veterinary medicine, benha university | ||||
| 5Genome Research unit, Animal Health Research Institute | ||||
| Abstract | ||||
| Camel pox is a viral contagious notifiable zoonotic disease causing variable rates of mortality, morbidity and cutaneous lesions in camels and humans. It caused by camelpox virus (CMLV), an orthopoxvirus and can be fatal in young camels. Confirmative, rapid and sensitive diagnostic techniques are required for accurate diagnosis of the disease, so in the present study, a duplex real-time PCR assay was developed for rapid detection of camelpox virus (CMLV) based on targeting both C18L and DNA pol genes. The detection limit was 0.4 pg and 4 pg of viral DNA for C18L and DNA pol genes, respectively. The duplex assay detected CMLV DNA in different types of spiked samplesand providesa rapid diagnostic tool for camel pox especiallyin borders where testing of large number of animals during importation and exportation is applied. Further evaluation of the assay on other clinical samples or samples from experimentally infected animal may be required. | ||||
| Keywords | ||||
| Camelpox; Camels; Duplex; Diagnosis; Real-time PCR | ||||
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