Evaluation of the anticancer activity of dipyridamole and Imatinib mesylate compounds against breast cancer cell line and related biochemical and genetic changes | ||||
Journal of Bioscience and Applied Research | ||||
Article 8, Volume 7, Issue 4, December 2021, Page 198-210 PDF (1.49 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jbaar.2021.209054 | ||||
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Authors | ||||
Rania A. El-Leithy1; Aly F. Mohamed2; Asmaa A. EL-refaay1; Mohamed M. Omran 1 | ||||
1Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt | ||||
2Former Head of R&D Sector, The Holding Company for Production of Vaccines, Sera and Drugs (VACSERA), Giza, Egypt | ||||
Abstract | ||||
Imatinib mesylate and dipyridamole were antineoplastic targeted chemotherapeutic agents, and it is here evaluating the anticancer of imatinib mesylate and dipyridamole compounds against MDA-MB231 breast cancer cell line in-vitro and the related cell cycle and gene profile. MDA-MB231 cells showed more sensitivity to imatinib mesylate than to dipyridamole, with an IC50 value of 348 g/mL versus 494 g/mL for imatinib mesylate and dipyridamole, respectively (P-value < 0.05). The up/downregulation of Bax and Bcl-2 and metastasis contributing gene (MMP-1) assured the anticancer activity. Also, the apoptotic potential of dipyridamole and imatinib mesylate was verified by arresting cells in the G2/M phase and increasing the percentage of the apoptotic cells in the pre-G1 phase. The antioxidant levels were drug dependent, as they were significantly higher(P<0.05) in cells treated with imatinib than in cells treated with Dipyridamole. Conclusion: Imatinib mesylate growth-inhibitory impact on breast cell lines, either alone or in combination with dipyridamole, may be mediated through up/downregulation of the Bax, Bcl-2, and MMP-1 genes. Imatinib is a promising treatment for breast cancer patients who require targeted therapy. | ||||
Keywords | ||||
Dipyridamole; imatinib mesylate; Breast cancer; apoptotic genes; cell cycle; antioxidants | ||||
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