Extraction and characterization of Antigenic S- lipopolysaccharides Brucella abortus S99.
Roushdy, C., Gouda, M., Moustafa, A., Ibrahim, F., El-bauomy, E., Fadel, M. (2021). Extraction and characterization of Antigenic S- lipopolysaccharides Brucella abortus S99.. EKB Journal Management System, 41(1), 154-158. doi: 10.21608/bvmj.2021.78132.1421
Cleopatra Mahmoud Roushdy; Mohamed Gouda; Abdel-Moneim Moustafa; Faysal Ibrahim; Essam El-bauomy; Mai A. Fadel. "Extraction and characterization of Antigenic S- lipopolysaccharides Brucella abortus S99.". EKB Journal Management System, 41, 1, 2021, 154-158. doi: 10.21608/bvmj.2021.78132.1421
Roushdy, C., Gouda, M., Moustafa, A., Ibrahim, F., El-bauomy, E., Fadel, M. (2021). 'Extraction and characterization of Antigenic S- lipopolysaccharides Brucella abortus S99.', EKB Journal Management System, 41(1), pp. 154-158. doi: 10.21608/bvmj.2021.78132.1421
Roushdy, C., Gouda, M., Moustafa, A., Ibrahim, F., El-bauomy, E., Fadel, M. Extraction and characterization of Antigenic S- lipopolysaccharides Brucella abortus S99.. EKB Journal Management System, 2021; 41(1): 154-158. doi: 10.21608/bvmj.2021.78132.1421

Extraction and characterization of Antigenic S- lipopolysaccharides Brucella abortus S99.

Benha Veterinary Medical Journal
Article 34, Volume 41, Issue 1, October 2021, Page 154-158  XML PDF (1.1 MB)
Document Type: Original Article
DOI: 10.21608/bvmj.2021.78132.1421
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Authors
Cleopatra Mahmoud Roushdy email 1; Mohamed Goudaorcid 2; Abdel-Moneim Moustafa2; Faysal Ibrahim3; Essam El-bauomy4; Mai A. Fadel5
1Department of Brucellosis, Animal Health Research Institute, Giza, Egypt
2Department of Infectious diseases, Faculty of Veterinary Medicine, Benha University, Egypt
3Department of Infectious diseases , Faculty of Veterinary Medicine, Benha University, Egypt
4Department of Brucellosis, Animal Health Research Institute, Agriculture Research Center
5Department Pharmacology and pyrogen unit, Department of chemistry, toxicology and feed deficiency, Animal Health Research Institute, Agriculture Research Center, P.O. Box 246, Giza 12618, Egypt
Abstract
Lipopolysaccharide (LPS)is the major valuable antigen used in diagnosis of brucellosis. In this study, to extract LPS from B. abortus strain 99 (reference strain), the hot-phenol method was used .Both chemical and biological characteristics were investigated to ensure quality and quantity of LPS to be involved in further diagnostic studies. Sugars represents about 50% of LPS (0.57 mg/ mg). HPLC analysis of the extracted LPS revealed that its purity was the same as of the commercial LPS of Salmonella Typhimurium. To determine the chemical configuration of S-type LPS, Proton-nuclear magnetic resonance (1H-NMR) was used. The extracted LPS could be cleaved typically to yield a lipid A and polysaccharide moieties (two portions) as main structure of Gram-negative bacteria. A glucosamine sugar residue expanded on a wide range region of NMR spectrum. Considering biological features, Rabbit Pyrogen Test (RPT) of extracted LPS showed low pyrogencity. While anti-complement activity of LPS was dose-dependent, high concentration caused high inhibition of hemolysis. Results emphasized the significance of these methods of extraction and characterization in obtaining valuable antigenic fraction LPS to be used in diagnosis or vaccination purposes.
Keywords
Lipopolysaccharide (LPS) / extraction; B. abortus S99; NMR; Rabbit pyrogen test (RPT); HPLC
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