SEQUENCE ANALYSIS OF K13 PROPELLER GENE POLYMORPHISM OF PLASMODIUM FALCIPARUM-INFECTED PATIENTS IN EGYPT | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Article 1, Volume 51, Issue 3, December 2021, Page 423-430 PDF (838.06 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jesp.2021.210411 | ||||
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| Authors | ||||
| SHAIMAA AHMED SHARAF-EL-DEEN; AHMED SAID TAWFIK MAHDY; OMIMA KAMEL ELSHAFEY; MOHAMED M. ABD EL-GHAFFAR; MANAL AHMED EL–MELEGY; AMANY IBRAHIM AMMAR | ||||
| Department of Parasitology, Faculty of Medicine, Menoufia University, Egypt | ||||
| Abstract | ||||
| Artemisinins (ART) are world's most effective anti-malarial drugs, which replaced chloroquine and other antimalarials after emergence of resisting strains to them. They caused a marked malaria deaths’ reduction with a hope of future world free of malaria. Unfortunately, Artemisinin-resisting Plasmodium strains have emerged in many Asian Countries, although rarely detected in Africa till now; regular screening of resistance genes is a must as Africa recorded the highest endemic and pandemic cases. So, emergence of ART resistance would be risky due to the limited treatment facilities. This study screened K13-propeller gene polymerphisms related to ART resistance in P. falciparum-infected patients at Abassia Fever Hospital, Egypt. Clinical and laboratory evaluations of patients were performed; those who displayed delayed parasite clearance were screened for polymorphisms of the K13-propeller gene by nested PCR and sequencing. Sequences were retrieved from 20 (90.9%) of the 22 samples with delayed clearance. Seven K13 gene mutations were discovered in 9 samples (9/20) (i.e., 45 %) of all tested sequences. Two of the seven-point mutations were synonymous, whereas the other five were non-synonymous (R410I, N772K, S775F, C778M, H783S) but not identified as a cause of delayed clearance. | ||||
| Keywords | ||||
| Egypt; Patients; Malignant malaria; Artemisin; K13-propeller gene polymerphisms | ||||
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