COMPARISON BETWEEN THE EFFECTS OF MAGNESIUM OXIDE (MGO) NANO-PARTICLES AND MICROWAVES (MVS) IRRADIATION ON GIARDIA LAMBLIA CYSTS | ||||
Journal of the Egyptian Society of Parasitology | ||||
Article 13, Volume 51, Issue 3, December 2021, Page 539-550 PDF (1.19 MB) | ||||
DOI: 10.21608/jesp.2021.210440 | ||||
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Authors | ||||
EMAN M. HUSSEIN1; ABDALLA M. HUSSEIN2; FARID EL-TANTAWY3; AMIRA B. MOKHTAR1; OLA A. ISMAIL1; EMAN K. EL-GAYAR1; WAFAA M. ZAKI1; REHAB I. ALI4; ASHRAF G. TEMSAH5 | ||||
1Department of Medical Parasitology, Faculty of Medicine, Suez Canal University | ||||
2Department of Physics, Faculty of Science, Al-Azhar | ||||
3Faculty of Science, Suez Canal University | ||||
4Faculty of Medicine Suez Canal University | ||||
5Faculty of Medicine, Al-Azhar, University | ||||
Abstract | ||||
The efficiency of the transmission of Giardia lamblia is assured by the presence of a cyst wall, which provides resistance to drastic osmotic and pH variations. Both magnesium oxide (MgO) nanoparticles (NPs) and microwaves (MVs) are known to inactivate many microorganisms. This study compared the effects of MgO NPs & MVs irradiation on Giardia lamblia cysts in experimental mice as to treated and un-treated cysts viability, excystation, ultrastructure changes and infectivity. The results showed that the G. lamblia cysts count were reduced to (LD90) after 8, 16, & 24hrs of exposure to doses 100, 50, & 25mg/ml, respectively, while reduction rate of (EC50) after 8, 16, &24 hrs of exposure to dose 50, 25, & 12.5mg/ml, respectively. MVs LD90 efficacy on cysts was detected after 30 seconds while EC50 after 20 seconds. Failure of excystation was 90% and 98% among MgO NPs and MVs treated cysts. MgO NPs treated cysts showed multiple cytoplasmic vesicular vacuoles while the cyst wall appeared destructive with the release of the cytoplasm outside. MVs treated cysts were filled with one big vesicular vacuole with an increase in size while some contents were decreased in size and others disappeared completely. The infectivity rate among mice inoculated with MgO NPs and microwave treated cysts was 33.3% & 8.2 % with a mild histopathological changes, with significant differences. | ||||
Keywords | ||||
Mice; Giardia lamblia; MgO NPs; Microwave; Infectivity; Viability; Ultrastructure | ||||
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