IDENTIFICATION AND GENOTYPES OF GIARDIA INTESTINALIS IN RUMINANT LIVESTOCK IN SOUTH SINAI GOVERNORATE, EGYPT | ||||
Journal of the Egyptian Society of Parasitology | ||||
Article 22, Volume 51, Issue 3, December 2021, Page 617-626 PDF (1.08 MB) | ||||
DOI: 10.21608/jesp.2021.210453 | ||||
View on SCiNiTO | ||||
Authors | ||||
SAFAA M. BARGHASH1; SAMIR A. TAHA2; SARA S. A. SERAG3; EHAB A. RAGAB4 | ||||
1Parasitology Unit, Department of Animal and Poultry Health, Animal Production and Poultry Division, Desert Research Center | ||||
2Department of Zoology, Department, Faculty of Science, Zagazig University | ||||
3Faculty of Science, Zagazig University | ||||
4Agricultural Economics Institute | ||||
Abstract | ||||
This study may be the first to detect genotype of Giardia among ruminants in South Sinai Governorate. Fresh iodine-fecal stained smears were examined by microscopy, followed by a nested PCR on frozen fecal specimens. The results showed Giardia in 40% (78/195) & 28.72% (56/195) of the examined ruminants by microscopic and nPCR, respectively. In cattle was (52.94%, 39.2%), in goats (37.5, 25.0%), and in sheep (34.82%, 25.0%). Animals <3 months old were more Giardia infected than older ones. Giardia infection was high in El Tur City (50%, 35.1%) than in Ras Sudr City (25.9%, 19.8%), and in males (51.3%, 35.5%) than in females (32.8%, 24.4%). Symptomatic animals were more susceptible to the infection than asymptomatic ones. Giardia was more prevalent in diarrheic samples than those with soft and hard nature with significant differences (P<0.05). Nested-PCR of G. intestinalis identified positive β-giardin gene band of 511 & 753bp. Analysis of five sharp generated fragments in both directions showed sequences with accession numbers MW925063, MW925066, & MW925067 (Assemblage A) & MW925064 & MW925065 (Assemblage B). It clustered with some Kenyan and Egyptian isolates with identity from 93.9% to 100% and genetic distance from 0.0 to 6.5 compared to other GenBank strains. Genotyping at sub-Assemblage level was essential for zoonotic transmission. | ||||
Keywords | ||||
Sinai; Giardia intestinalis; nested-PCR; beta-giardin (bg); Gene sequencing; Ruminants | ||||
Statistics Article View: 87 PDF Download: 187 |
||||