The possible ameliorative effect of vitamin D3 and/or omega-3 fatty acids in a rat model of type I early diabetic nephropathy: A physiological and histological study
El-Sokkary, N., Yusuf, A., Abdel-Moneim, R., Abdulrazeg, H., Hassaan, P. (2022). The possible ameliorative effect of vitamin D3 and/or omega-3 fatty acids in a rat model of type I early diabetic nephropathy: A physiological and histological study. EKB Journal Management System, 42(1), 101-117. doi: 10.21608/besps.2021.89323.1107
Nahed H. El-Sokkary; Alaaeldin Yusuf; Rehab A. Abdel-Moneim; Hanan Salah Abdulrazeg; Passainte S. Hassaan. "The possible ameliorative effect of vitamin D3 and/or omega-3 fatty acids in a rat model of type I early diabetic nephropathy: A physiological and histological study". EKB Journal Management System, 42, 1, 2022, 101-117. doi: 10.21608/besps.2021.89323.1107
El-Sokkary, N., Yusuf, A., Abdel-Moneim, R., Abdulrazeg, H., Hassaan, P. (2022). 'The possible ameliorative effect of vitamin D3 and/or omega-3 fatty acids in a rat model of type I early diabetic nephropathy: A physiological and histological study', EKB Journal Management System, 42(1), pp. 101-117. doi: 10.21608/besps.2021.89323.1107
El-Sokkary, N., Yusuf, A., Abdel-Moneim, R., Abdulrazeg, H., Hassaan, P. The possible ameliorative effect of vitamin D3 and/or omega-3 fatty acids in a rat model of type I early diabetic nephropathy: A physiological and histological study. EKB Journal Management System, 2022; 42(1): 101-117. doi: 10.21608/besps.2021.89323.1107

The possible ameliorative effect of vitamin D3 and/or omega-3 fatty acids in a rat model of type I early diabetic nephropathy: A physiological and histological study

Bulletin of Egyptian Society for Physiological Sciences
Article 9, Volume 42, Issue 1, January 2022, Page 101-117  XML PDF (1.02 MB)
Document Type: Original Article
DOI: 10.21608/besps.2021.89323.1107
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Authors
Nahed H. El-Sokkaryorcid 1; Alaaeldin Yusuf1; Rehab A. Abdel-Moneimorcid 2; Hanan Salah Abdulrazeg1; Passainte S. Hassaan email orcid 1
1Department of Medical Physiology, Faculty of Medicine, University of Alexandria, Alexandria, Egypt
2Department of Histology and Cell Biology, Faculty of Medicine, University of Alexandria, Alexandria, Egypt
Abstract
Diabetic nephropathy (DN) is one of the most common causes of chronic kidney diseases (CKDs) that have been associated with high morbidity and mortality. Recently, several studies have highlighted the protective role of vitamin D3 (VD3) and omega-3 fatty acids (O3-FAs) in CKDs. However, their effect on DN is still unclear. Aims: This study aimed to evaluate the effects of both VD3 and / or O3-FAs in slowing the progression of DN through their impact on indices of renal function, glycemic control, oxidative stress and markers of podocyte injury. Materials and methods: type I diabetes was induced in albino rats by single intraperitoneal injection of streptozotocin (65mg/kg). Rats received daily oral administration of VD3 and O3-FAs separately and in combination for 6 weeks. Results: VD3 and O3-FAs therapy improved significantly hyperglycemia, renal function tests, with concomitant decrease in total urinary protein content, urinary nephrin, a marker of podocyte injury and renal oxidative stress. However, the combined therapy was superior in its effect over VD3 and O3-FAs separately. Such results were confirmed by renal cortical tissue assessment using light microscopic examination of H&E and PAS stains in addition to transmission-electron microscopy. Conclusions: VD3 and O3-FAs have a potential beneficial effect on amelioration of structural changes in podocytes and glomerular basement membrane that was reflected as evident renal functional restoration.
Keywords
Diabetic nephropathy; vitamin D; omega-3 fatty acids; nephrin
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