DNA Barcoding for Identification of Some Fish Species (Sparidae) in Mediterranean Sea Area
Eissa, S., Fiteha, Y., Elhifnawy, H., Magdy, M., Mamoon, A., Hussien, N., Rashed, M. (2021). DNA Barcoding for Identification of Some Fish Species (Sparidae) in Mediterranean Sea Area. EKB Journal Management System, 38(part 2 (Biological Sciences)), 168-182. doi: 10.21608/jsrs.2021.210686
Shaimaa Mohamed Eissa; Yosur Gamal Fiteha; Hagar Tarek Elhifnawy; Mahmoud Magdy; Ahmed Mamoon; Nadia Hussien; Mohamed Abdel-Salam Rashed. "DNA Barcoding for Identification of Some Fish Species (Sparidae) in Mediterranean Sea Area". EKB Journal Management System, 38, part 2 (Biological Sciences), 2021, 168-182. doi: 10.21608/jsrs.2021.210686
Eissa, S., Fiteha, Y., Elhifnawy, H., Magdy, M., Mamoon, A., Hussien, N., Rashed, M. (2021). 'DNA Barcoding for Identification of Some Fish Species (Sparidae) in Mediterranean Sea Area', EKB Journal Management System, 38(part 2 (Biological Sciences)), pp. 168-182. doi: 10.21608/jsrs.2021.210686
Eissa, S., Fiteha, Y., Elhifnawy, H., Magdy, M., Mamoon, A., Hussien, N., Rashed, M. DNA Barcoding for Identification of Some Fish Species (Sparidae) in Mediterranean Sea Area. EKB Journal Management System, 2021; 38(part 2 (Biological Sciences)): 168-182. doi: 10.21608/jsrs.2021.210686

DNA Barcoding for Identification of Some Fish Species (Sparidae) in Mediterranean Sea Area

Journal of Scientific Research in Science
Article 8, Volume 38, part 2 (Biological Sciences), January 2021, Page 168-182  XML PDF (653.79 K)
Document Type: Original Article
DOI: 10.21608/jsrs.2021.210686
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Authors
Shaimaa Mohamed Eissa email 1; Yosur Gamal Fiteha2; Hagar Tarek Elhifnawy3; Mahmoud Magdy3; Ahmed Mamoon4; Nadia Hussien2; Mohamed Abdel-Salam Rashed3
1Zoology, women collage, Ain shams universty
2Faculty of Women for Arts, Science and Education, Zoology Department, Ain Shams University, Cairo, Egypt.
3Faculty of Agriculture, Genetics department, Ain Shams University, Cairo, Egypt.
4Faculty of Agriculture, Fish production department, Al Azhar University, Cairo, Egypt.
Abstract
Background: DNA barcoding depend on a piece of the cytochrome C oxidase subunit I (COI) gene in the mitochondrial genome is broadly useful in species ID and biodiversity studies. The aim of this study is to create a complete barcoding reference database of some fishes in the Mediterranean Sea fall under the family Sparidae. . Materials and methods: Mitochondrial COI barcode sequences were demonstrated from 8 species of family Sparidae in the order Perciformes, the mean length of Mitochondrial COI sequences was 650 base pairs. Results: The results of the phylogenetic tree presented that monophyly of Sparidae species. The studied species displayed clades of conspecific sequences and showed a match between the present study and the GenBank (NCBI) database. All groups clustered with high bootstrap value, that showed next to each branch and the tree was rooted based on the out group of Rhincodon typus. Conclusion: We achieve that COI sequencing can be used to recognize different fish species, and also it is used to obtain high competence of species reorganization by DNA barcoding. We underline the power of DNA barcode and its tools to identify different species from Mediterranean Sea. Results give Species ID for each species under study by using DNA barcoding.
Keywords
DNA; Barcoding; Mediterranean; Fish; Sparidae; PCR; COI
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