The impact of addition of ascorbic acid to cryopreservation medium on dog epididymal spermatozoa | ||||
| Veterinary Medical Journal (Giza) | ||||
| Article 14, Volume 67, Issue 1, 2021, Page 161-173 PDF (306.77 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmjg.2021.210948 | ||||
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| Authors | ||||
Eman Fayez1; Ali Salama  1; Zaher M Rawash2; Mohamed A. I. El Sayed1
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| 1Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt | ||||
| 2Animal Reproduction Research Institute, Giza, Egypt | ||||
| Abstract | ||||
| The objective of this study was to assess the effect of the addition of different concentrations of ascorbic acid to the extender on frozen-thawed epididymal dog spermatozoa. Epididymides from 17 castrated dogs were minced and incubated in a Tris buffer. The retrieved spermatozoa were diluted with Tris based-egg yolk-glycerol extender supplemented with different concentrations of ascorbic acid (0.45 mg/ml and 0.90 mg/ml) and the control (0.0 mg/ml). Diluted samples were equilibrated at 5°C for 2 h, packaged in 0.25 ml straws, and stored in liquid nitrogen (-196°C). After thawing (37°C for 30 s), sperm motility, viability, membrane integrity, acrosome integrity, DNA integrity, and lipid peroxidation by malondialdehyde (MDA) concentration were evaluated. The results were expressed as mean ± SE. Adding 0.90 mg/ml ascorbic acid to the cryopreservation medium significantly (P<0.05) improved motility, viability, membrane and acrosome integrity compared to the control. MDA concentration was significantly (P<0.05) reduced at 0.90 mg/ml related to the control. Percent of DNA damage was significantly (P<0.05) reduced in 0.45 mg/ml and 0.90 mg/ml ascorbic acid compared to the control. In conclusion, addition of ascorbic acid (0.90 mg/ml) to TCF extender resulted in a significant increase in the percentage of motility, viability, membrane intact, and acrosome- intact canine epididymal sperm, as well as the maintenance of DNA integrity and the reduction of lipid peroxidation at the membrane level. | ||||
| Keywords | ||||
| Dog; Epididymal sperm; Cryopreservation; Ascorbic acid; oxidative stress | ||||
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