The Protective Impact of Lycopene and Folic Acid Supplementation Against Nicotine Toxicity on Pancreatic Islets in Adult Male Albino Rats: Biochemical and Immuno-Histochemical Study | ||||
| Egyptian Journal of Histology | ||||
| Article 3, Volume 46, Issue 2, June 2023, Page 518-532 PDF (2.65 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2021.106915.1592 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
mai Ibrahim  1; nehal fahmy shaheen2
| ||||
| 1Anatomy & Embryology department, Faculty of medicine, Benha university, Benha, Egypt. | ||||
| 2Anatomy&Embryology department,Faculty of medicine,Benha University | ||||
| Abstract | ||||
| Introduction: It is reported that dysfunctioning of islets and elevated levels of fasting blood glucose are found in rats exposed to nicotine. Lycopene is thought to have a potential role as an effective antioxidant in the prevention of chronic diseases associated with oxidative stress. Folic acid is a water‐soluble vitamin B that is essential for amino acid metabolism. Aim of the Study: To explore the effects of nicotine toxicity on pancreatic islets and protective impact of lycopene & folic acid supplementation. Material and Methods: Fifty healthy adult male albino rats were separated into five groups. Control , Nicotine treated at which, rats were injected intraperitoneal by nicotine 3mg/kg daily for a period of 3 weeks . Nicotine + Lycopene treated group at which rats received lycopene at a dosage of 10 mg/kg b.wt. daily in combination with nicotine treatment for 3 weeks. Nicotine + Folic acid treated group at which ,rats were injected nicotine and given Folic acid orally at a dosage of 36 μg/kg. b.wt and recovery group at which rats kept for one month after 3 weeks of nicotine injection. then pancreatic tissues were examined for histopathological and immunohistochemical changes. Results: Nicotine treated group showed degenerated pancreatic islets with ill-defined outline . Numerous collagen fibers were present within and around the pancreatic islets in masson stained pancreatic sections, Strong INOs immunoreactivity but anti-insulin immuno expression has decreased. Lycopene and Folic acid reduces toxic effect of nicotine on pancreatic islets, but Folic acid revealed a significant decrease in collagen fibers, INOs immunoreactivity and significant increase in anti-insulin immuno expression compared with that in Lycopene group. Conclusion: Use of lycopene during the period of nicotine injection considered to have a protecting influence on pancreatic islets. Meanwhile The use of Folic acid has a more protection than Lycopene. | ||||
| Keywords | ||||
| Folic acid and pancreatic islets; lycopene; nicotine | ||||
|
Statistics Article View: 379 PDF Download: 349 |
||||
