Acinetobacter baumannii extracted silver nanoparticles: A study on the characterization and antibacterial activity | ||||
| Microbes and Infectious Diseases | ||||
| Article 16, Volume 3, Issue 1, February 2022, Page 135-148 PDF (767.4 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2021.109802.1212 | ||||
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| Authors | ||||
Mona Abdelhamid1; Rateb Nabil Abass2; Magdy Kamal Khalil3; A. B. Abeer Mohammed4; fatma O khalil   5
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| 1Clinical pharmacist. National Liver Institute, Menoufia University | ||||
| 2Associate Professor of Microbial Genetics, Microbial Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt | ||||
| 3Professor of Anesthesia and Intensive Care, National Liver Institute, Menoufia University, Shebin El-kom 32511, Egypt | ||||
| 4Lecturer of Microbial Biotechnology, Microbial Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt | ||||
| 5Microbiology nd Immunology, National Liver Institute, Menoufia University, Shebin El-kom, Egypt | ||||
| Abstract | ||||
| Background: Antibiotic resistance is a worldwide problem, associated with increased mortality. Biosynthesized silver nanoparticles are promising solution. Aim: To improve the anti-microbial resistance using Acinetobacter baumannii (A. baumannii) biosynthesized silver nitrate.Methods: Optimization of biosynthesis condition and characterization by Ultraviolet-Visible spectroscopy (U-V-Vis) at a wavelength from 300 to 400 nm, ±1.67 nm. The Acinetobacter baumannii biosynthesized silver nitrate was examined using transmission electron microscope (TEM). Fourier transformed infrared spectroscopy was done to study the capping material, X-ray spectroscopy confirm silver nanoparticles synthesis with particle size match TEM measuring and testing its antibacterial effect was done. Results: The obtained U.V Spectroscopy and TEM characters of silver nitrate biosynthesized A.baumannii. Acinetobacter baumannii biosynthesized silver nitrate was obtained indicated. Minimum inhibitory concentration (MIC) was from 1-3 mg/ml against extreme drug resistance bacteria. the size of silver nanoparticles was found to be between 18 nm and 50 nm. The AgNPs were found to be stable for over 6 months at 37°C.The MIC for AgNPs formed from A. baumannii CFF was detected as 2.85 mg/ml, MBC (minimal bactericidal concentration) was 3 mg/ml for K. pneumonia, MIC was 1 mg/ml, MBC was 0.5 mg/ml for P. aeruginosa and MIC was 1.5 mg/ml and MBC was 1 mg/ml for A. baumannii isolates. Lethal concentration (LC) 50% was 60.584 ug/ml) assessed by Sulforhodamine-B assay on hepatocellular carcinoma cell line. Conclusion: Acinetobacter baumannii extracted silver nitrate has A promising antibacterial action that may help in solving the multidrug resistance bacteria. | ||||
| Keywords | ||||
| Silver nanoparticles; antibiotic resistance; Acinetobacter baumannii; cytotoxicity; hepatocellular carcinoma | ||||
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