Effect of Copper Sulphate Pollution and its Antidote Penicillamine on Liver and Serum Markers of Albino Rats | ||||
| Zagazig Veterinary Journal | ||||
| Article 5, Volume 49, Issue 4, December 2021, Page 431-443 PDF (544.7 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/zvjz.2021.95736.1155 | ||||
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| Authors | ||||
Medhat Fawzy1; Sara Ahmed  1, 2; Tarek Khamis3; Ahmed Arisha 4, 5; doaa Abdel-Fattah1
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| 1Biochemistry Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt | ||||
| 2Bachelor of Pharmacy, Faculty of Pharmacy, Zagazig University, 44519, Zagazig, Egypt | ||||
| 3Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt | ||||
| 4Department of Animal Physiology and Biochemistry, Faculty of Veterinary Medicine, Badr University, Badr City, Cairo, Egypt | ||||
| 5Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt | ||||
| Abstract | ||||
| Copper is an essential trace element and is required for many metabolic functions. The present study was designed to study the negative impact of excess copper sulphate (Cuso4) on liver of albino rats and studying how its antidote d-penicillamine play role in the development of its side effects. Seventy albino rats were divided into seven equal groups each containing 10 rats (G1:control group received distilled water) ; (G2: 0.1 LD50 of CuSO4) ; (G3: 0.2 LD50 of CuSO4) ; (G4: 0.4 LD50 of CuSO4) ; (G5: 0.1 LD50 of CuSO4 +100 mg/kg/day of penicillamine) ; (G6: LD50 of CuSO4.+100 mg/kg/day of penicillamine) and (G7: LD50 of CuSO4+ 100 mg/kg/day of penicillamine) for 30 days. At the end of the experiment all rats were sacrificed, and blood samples and liver tissues were collected for biochemical and molecular assaying. The result showed that administration of copper sulphate with different levels induced a significant increase in fasting blood glucose level (FBG), lipid peroxidation marker (MDA), serum copper level, white blood cells (WBCs) and serum tyrosinase activity, but a significant decrease in total antioxidant activity (TAC), Hb and platelet count. Moreover, copper sulphate administration elicited a significant (P ˂ 0.05) downregulation of cytochrome c oxidase (Cyto co) and glucose -6- phosphate dehydrogenase (G6PD). It could be approved that d- penicillamine (DPA) can decrease the negative impact of copper sulphate on hepatic tissues and serum enzymes. DPA can reduce hepatotoxicity and oxidative stress caused by copper pollution. | ||||
| Keywords | ||||
| Heavy metals; D- Penicillamine; Copper sulphate pollution; Albino rats; Gene expression | ||||
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