Evaluation of Ascitic Fluid Calprotectin as an Accurate Marker for Rapid Diagnosis of Spontaneous Bacterial Peritonitis in Patients with Chronic Liver Disease
Shafik, A., Elgazzar, A., Elgazar, A., Mostafa, M., Elaraby, M. (2022). Evaluation of Ascitic Fluid Calprotectin as an Accurate Marker for Rapid Diagnosis of Spontaneous Bacterial Peritonitis in Patients with Chronic Liver Disease. EKB Journal Management System, 31(1), 47-54. doi: 10.21608/ejmm.2022.211949
Ahmed O. Shafik; Ahmed M. Elgazzar; Ahmed S. Elgazar; Mysa S. Mostafa; Marwa Elaraby. "Evaluation of Ascitic Fluid Calprotectin as an Accurate Marker for Rapid Diagnosis of Spontaneous Bacterial Peritonitis in Patients with Chronic Liver Disease". EKB Journal Management System, 31, 1, 2022, 47-54. doi: 10.21608/ejmm.2022.211949
Shafik, A., Elgazzar, A., Elgazar, A., Mostafa, M., Elaraby, M. (2022). 'Evaluation of Ascitic Fluid Calprotectin as an Accurate Marker for Rapid Diagnosis of Spontaneous Bacterial Peritonitis in Patients with Chronic Liver Disease', EKB Journal Management System, 31(1), pp. 47-54. doi: 10.21608/ejmm.2022.211949
Shafik, A., Elgazzar, A., Elgazar, A., Mostafa, M., Elaraby, M. Evaluation of Ascitic Fluid Calprotectin as an Accurate Marker for Rapid Diagnosis of Spontaneous Bacterial Peritonitis in Patients with Chronic Liver Disease. EKB Journal Management System, 2022; 31(1): 47-54. doi: 10.21608/ejmm.2022.211949

Evaluation of Ascitic Fluid Calprotectin as an Accurate Marker for Rapid Diagnosis of Spontaneous Bacterial Peritonitis in Patients with Chronic Liver Disease

Egyptian Journal of Medical Microbiology
Article 7, Volume 31, Issue 1, January 2022, Page 47-54  XML PDF (401.87 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2022.211949
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Authors
Ahmed O. Shafik1; Ahmed M. Elgazzar1; Ahmed S. Elgazar2; Mysa S. Mostafa1; Marwa Elaraby email 3
1Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt
2Department of Hepatology and Gastroentrology, Faculty of Medicine, Benha University, Egypt.
3Department of Medical Microbiology and immunology, Faculty of Medicine, Benha University, Egypt.
Abstract
Background: Spontaneous bacterial peritonitis is the development of an infection of the ascitic fluid in the peritoneum, with no identifiable source of the infection in patients with liver failure. ascitic fluid neutrophil count is more than 250⁄mm3. calprotectin in Ascitic fluid may help in detection of neutrophil count < 250 cells/mm3, it has an effective role in detection of SBP and this will be a rapid treatment and good prognosis. Objectives: Evaluation of  the calprotectin in ascitic fluid  as  rapid  marker for diagnosis of SBP. Methodology: our study was done on 40 patients in Hepatology and Gastroenterology Department of Benha University divided into 2 groups 20 patients were SBP group and other 20 patients were non SBP group. 5ml of ascitic fluid was collected from  patient in sterile blood culture bottles under complete aseptic condition then cultured on automated blood culture system (Bact/ALERT 3D) .Serum Calprotectin was measured in ascitic  fluid by using commercially available  quantitative sandwich enzyme-linked  immune sorbent assays. Results: SBP was in significant association with higher TLC in AF and higher frequency of positive culture results when compared to non SBP group (p < 0.001 for each).Among all studied cases, median calprotectin level was 0.602 ng/dL. SBP was significantly associated with higher level of calprotectin when compared to non SBP group .Calprotectin showed positive significant correlation with TLC in ascitic fluid. Conclusion: calprotectin in ascitic fluid is an excellent rapid  marker for diagnosis of spontaneous bacterial peritonitis in patients with chronic Liver disease.
Keywords
Spontaneous bacterial peritonitis; Calprotectin; ELISA
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