Detection of Human Parvovirus B19 Infection in Cases of Systemic Lupus Erythematosus Attending Benha University Hospitals
Shafik, A., El-Gazar, A., El-Nahal, A., Ibrahim, N., El-Sayed, R. (2022). Detection of Human Parvovirus B19 Infection in Cases of Systemic Lupus Erythematosus Attending Benha University Hospitals. EKB Journal Management System, 31(1), 105-111. doi: 10.21608/ejmm.2022.212000
Ahmed O. Shafik; Ahmed M. El-Gazar; Ayat shaban El-Nahal; Noha H. Ibrahim; Rasha A. El-Sayed. "Detection of Human Parvovirus B19 Infection in Cases of Systemic Lupus Erythematosus Attending Benha University Hospitals". EKB Journal Management System, 31, 1, 2022, 105-111. doi: 10.21608/ejmm.2022.212000
Shafik, A., El-Gazar, A., El-Nahal, A., Ibrahim, N., El-Sayed, R. (2022). 'Detection of Human Parvovirus B19 Infection in Cases of Systemic Lupus Erythematosus Attending Benha University Hospitals', EKB Journal Management System, 31(1), pp. 105-111. doi: 10.21608/ejmm.2022.212000
Shafik, A., El-Gazar, A., El-Nahal, A., Ibrahim, N., El-Sayed, R. Detection of Human Parvovirus B19 Infection in Cases of Systemic Lupus Erythematosus Attending Benha University Hospitals. EKB Journal Management System, 2022; 31(1): 105-111. doi: 10.21608/ejmm.2022.212000

Detection of Human Parvovirus B19 Infection in Cases of Systemic Lupus Erythematosus Attending Benha University Hospitals

Egyptian Journal of Medical Microbiology
Article 15, Volume 31, Issue 1, January 2022, Page 105-111  XML PDF (587.57 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2022.212000
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Authors
Ahmed O. Shafik1; Ahmed M. El-Gazar1; Ayat shaban El-Nahal email 2; Noha H. Ibrahim3; Rasha A. El-Sayed1
1Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt
2medical microbiology and immunology ,faculty of medicice ,kafr el sheik university
3Department of Rheumatology, Rehabilitation and Physical medicine Faculty of Medicine, Benha University, Egypt
Abstract
Background: Systemic lupus erythematosus is an autoimmune disease. Parvovirus B19 infection can break tolerance to self-DNA and promote pathogenesis of autoimmunity and might induce either idiopathic SLE in a person who is genetically susceptibl or it might induce a SLE-like picture. Objectives: Detection of PV-B19 DNA and it's IgM and IgG antibodies in the serum of SLE patients and in apparently healthy volunteer in Benha University Hospitals. Methodology: The study was conducted  on 60 subjects classified into 2 groups: Group I: Including 40 SLE patients fulfilling SLICC Classification Criteria , 20 of them  were in exacerbation  state   and the other  20  cases were  in remission state and  Group II: Including 20 age and sex matched  apparently healthy volunteers serve as a control group . All patients were subjected to full history taking, clinical examination and laboratory investigations. PV- B19 IgM and IgG were measured using anti PV- B19 ELISA kits and PV-B19 DNA was detected by using real timepolymerase chain reaction(PCR). Results: PV- B19 DNA was detected in 9 cases (45%)  with active disease,  Out of the 9 patients with  PV- B19 DNA, only 1 had positive PCR  results alone ,  1 had positive PCR and IgM results  and  7  had positive  PCR, IgM and IgG results. on the other hand, PV- B19 DNA was detected in only 1 case  (5%) out of   20 remittent cases (p=0.003). Active cases were significantly associated with higher frequency of IgM and IgG when compared to remittent cases (p=0.010, 0.020, respectively).There was significant differences in SLEDAI between active and remitted cases (p < 0.001). Conclusion: Combined use of PCR and ELISA are needed for  accurate diagnosis of  PV- B19 infection.
Keywords
Systemic lupus erythematosus; Parvovirus B19 IgM and IgG; Parvovirus B19-DNA detection by PCR
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