Assessment the effect of Cisplatin and Doxorubicin on Nitric Oxide levels on HepG2 cell line

Abdel-Hamid, Nabil Mohie; EL-Gharieb, Mohammed Samy; El- Senduny, Fardous Fawzy; Alnakib, Nadia

doi:10.21608/ajbas.2022.104013.1074

	Assessment the effect of Cisplatin and Doxorubicin on Nitric Oxide levels on HepG2 cell line
Alfarama Journal of Basic & Applied Sciences
Article 3, Volume 3, Issue 2, July 2022, Page 220-229 PDF (492.34 K)
Document Type: Original Article
DOI: 10.21608/ajbas.2022.104013.1074
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Authors
Nabil Mohie Abdel-Hamid¹; Mohammed Samy EL-Gharieb²; Fardous Fawzy El- Senduny³; Nadia Alnakib ⁴
¹professor of biochemistry, faculty of pharmacy, kafr-elsheikh university
²Biochemistry Department, Faculty of Sciences, Port-Said University
³Assist Prof. of Biochemistry and Molecular Biology, Faculty of Sciences, MansouraUniversity
⁴Chemistry department, Faculty of Science, Port Said University, Egypt.
Abstract
Background: Mortality due to HCC is the fourth most common cause of mortality among Americans. Furthermore, HCC is most common in Asia and Africa, where hepatitis C and B are prevalent. Hepatocellular cancer therapy relies heavily on chemotherapy, from between the chemotherapy medicines cisplatin and doxorubicin, two of the most widely prescribed for the treatment of liver cancer. Aim of the study: To determine the cytotoxicity of cisplatin and doxorubicin in HepG2 cell line by the methyl thiazole tetrazolium (MTT) assay and assess nitric oxide (NO) levels and their significance as oxidative status markers in the progression of HCC. Material and Methods: HepG2 cells were incubated with different drugs concentrations. The half-maximal inhibitory concentration (IC50) values were determined the methyl thiazole tetrazolium (MTT) for cisplatin and doxorubicin. Results: IC50 values for HepG2 were found for doxorubicin and cisplatin (1.679 μg and 4.323 μg ), respectively. The NO levels in the HepG2 cell line treated with Cisplatin and Doxorubicin were measured. We found limited elevated NO levels after treating HepG 2 cell lines by doxorubicin. Moreover, there was significant inhibition in NO levels after treating with cisplatin. Conclusion: The findings suggest that nitric oxide levels can be utilized as a new diagnostic marker with predictive value for HCC.
Keywords
Doxorubicin; Cisplatin; Hepatocellular carcinoma; Nitric oxide; MTT assay


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