TRIALS FOR PREPARATION OF COMBINED INACTIVATED VACCINE AGAINST RESPIRATORY VIRAL DISEASES OF POULTRY (ND-IB-ILT) | ||||
| Egyptian Journal of Agricultural Research | ||||
| Article 25, Volume 85, Issue 1, March 2007, Page 307-316 PDF (2.2 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejar.2007.213781 | ||||
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| Author | ||||
| MOHAMED A. A. ABD EL-KHALECK | ||||
| Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt | ||||
| Abstract | ||||
| One hundred and seventy susceptible chicks, 21 days old, were divided into four main groups. The 15`, 2nd and 3`d groups were vaccinated by three different. designed combined oil inactivated vaccines against Newcastle (ND), Infectious Bronchitis (IB) and Infectious Laryngo-tracheitis (ILT) diseases viruses while the 4thgroup was kept as control. Half of each vaccinated group was boostered three weeks later by the corresponding prepared vaccine. Blood and serum samples were collected for evaluation of cellular and humoral immune responses. Estimation of cell mediated immunity was carried out using lymphocyte blastogenesis and phagocytic activity. Better response was obtained in the Pt group at 2 weeks post initial vaccination and 1 week after boostering. There is a decrease in the heterophil/lymphocyte ratio in group 1 in comparison with the highest ratio in control group. The humoral immune response was detected by haemagglutination inhibition test for NDV,,serum neutralization test (SNT) and ELISA test for IBV and SNT for ILT virus. The highest value of the antibody titre was recorded for the rt designed vaccine followed by the 2nd designed vaccine and finally the 3rd designed vaccine. | ||||
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