Pollen Morphology and Protein Pattern of Nitraria retusa and Some Selected Taxa of Zygophyllaceae in Egypt | ||||
| Egyptian Journal of Botany | ||||
| Article 4, Volume 55, Issue 2, April 2015, Page 207-230 PDF (2.41 MB) | ||||
| Document Type: Regular issue (Original Article) | ||||
| DOI: 10.21608/ejbo.2015.214 | ||||
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| Abstract | ||||
| POLLEN morphology and pattern of <i>Nitraria retusa</i> (Forssk.) Asch. were studied and compared against eight selected taxa of Zygophyllaceae viz. Fagonia arabica,<i> F. cretica, Peganum harmala, Tribulus terrestis, Zygophyllum album, Z. coccenium, Z. decumbens and Z. simplex</i>using Scanning Electron Microscope (SEM) and sodium dodecyle sulphate-polyacrylamide gel electrophoresis technique (SDS-PAGE). A wide range of measurements were obtained from digitized SEM images of whole pollen grains and exine pattern. Twenty nine protein bands were obtained with 100% polymorphism among the species examined.<i>F. arabica </i> and<i> T. terrestis </i>were characterized by one positive unique band with a molecular weight 56.8 and 24 kDa respectively.<i>P. harmula</i> was characterized by two positive unique bands with a molecular weight 62 and 42 kDa. N. retusa was characterized by three positive unique bands with amolecular weigh 64.6, 60.7 and 36 kDa. A dendrogram was constructed based on the similarity data matrix by unweighted pair group method using arithmetic averages cluster analysis. SEM and protein pattern analysis recommended the separation of <i>Nitraria retusa</i> (Forssk.) Asch. besides Fagonia sp. into a distinct family. | ||||
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