In Vitro Cyanobacterial Isolates Growth and their Effect on Rice Crop Cultivated in Pesticide-Treated Fields | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 1, Volume 12, Issue 12, December 2021, Page 211-218 PDF (676.42 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2021.214572 | ||||
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| Authors | ||||
H. A. H. EL-Zawawy1; S. El-Kadi2; Dina F. I. Ali  3
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| 1Botany Dept. (Microbiology) Fac. of Agric. Al-Azhar Univ. Cairo, Egypt. | ||||
| 2Agricultural Microbiology Department, Faculty of Agriculture, Damietta University, Damietta, Egypt. | ||||
| 3Agricultural Microbiology Department, Faculty of Agriculture, Mansoura University, Mansoura, Egypt. | ||||
| Abstract | ||||
| An experiment was carried out in Sakha Research Station, Kafrelsheikh governorate, Egypt during the 2018-2019 seasons to isolate the cyanobacteria dominated in the soil samples of rice fields polluted by two pesticides (carbofuran and penoxsulam). These cyanobacterial isolates were identified and the best isolates, regarding growth activities and nitrogen (N2) fixation capacities, were chosen and further applied to the rice field to study their effect on rice yield under different urea concentrations (0, 35 and 69 kg N2/fed). Data revealed that nine cyanobacterial isolates were successfully obtained and purified. A growth curve experiment was conducted on these isolates and revealed that three isolates (Nostoc muscorum, Anabaena cylindrica, and Anabaena variabilis) had the best growth activities and capacities for N2 fixation. Within limit, both pesticides showed stimulation of both cell growth and N2 fixation. With higher concentrations, these values tended to decline due to the possible inhibitory or toxic effect of these pesticides. Generally, cyanobacterial isolates were less tolerant to penoxsulam and were inhibited at lower concentrations compared to carbofuran. With carbofuran application on rice field (Egyptian hybrid rice 1 variety), the interaction between cyanobacteria and urea resulted in the highest grain yield with the recommended dose of urea (69 kg N2/fed), while the highest 1000-grain weight was obtained with half the recommended dose of urea (35 kg N2/fed). Moreover, the use of a mixture of cyanobacterial species rather than a single one resulted in more satisfactory results. | ||||
| Keywords | ||||
| Cyanobacteria; Nitrogen fixation; Nostoc; Anabaena; Pesticides; Rice crop | ||||
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