The relationship between the gut microbiota shifts and the inflammatory biomarkers in obese and normal weight adults
Mohammed, S., Hosny, M., Mohamed, W., Ezz Eldeen, M., Badary, M. (2022). The relationship between the gut microbiota shifts and the inflammatory biomarkers in obese and normal weight adults. EKB Journal Management System, 3(3), 703-713. doi: 10.21608/mid.2022.113960.1223
Shereen Mohammed; Marwa Fayez Hosny; Wegdan Mohamed; Manal Elsayed Ezz Eldeen; Mohamed Saad Badary. "The relationship between the gut microbiota shifts and the inflammatory biomarkers in obese and normal weight adults". EKB Journal Management System, 3, 3, 2022, 703-713. doi: 10.21608/mid.2022.113960.1223
Mohammed, S., Hosny, M., Mohamed, W., Ezz Eldeen, M., Badary, M. (2022). 'The relationship between the gut microbiota shifts and the inflammatory biomarkers in obese and normal weight adults', EKB Journal Management System, 3(3), pp. 703-713. doi: 10.21608/mid.2022.113960.1223
Mohammed, S., Hosny, M., Mohamed, W., Ezz Eldeen, M., Badary, M. The relationship between the gut microbiota shifts and the inflammatory biomarkers in obese and normal weight adults. EKB Journal Management System, 2022; 3(3): 703-713. doi: 10.21608/mid.2022.113960.1223

The relationship between the gut microbiota shifts and the inflammatory biomarkers in obese and normal weight adults

Microbes and Infectious Diseases
Article 22, Volume 3, Issue 3, August 2022, Page 703-713  XML PDF (794.07 K)
Document Type: Original Article
DOI: 10.21608/mid.2022.113960.1223
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Authors
Shereen Mohammed email orcid 1; Marwa Fayez Hosny2; Wegdan Mohamed1; Manal Elsayed Ezz Eldeen3; Mohamed Saad Badary4
1Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt
2Medical Microbiology and Immunology department, Faculty of Medicine, Assiut University, Assiut, Egypt
3Internal Medicine department, Faculty of Medicine, Assiut University, Assiut, Egypt
4Medical Microbiology and Immunology department, Faculty of Medicine, Assiut University , Assiut, Egypt
Abstract
Background:Healthy obesity means obese individuals remain free of metabolic health complications. Several researches link microbiota changes to obesity, focus on metabolic role of IL-6 and its ability to alter fat metabolism and correlate circulating calprotectin concentration to morbid obesity. This study evaluated the relative abundance of gut microbiota (Prevotella and γ-Proteobacteria), estimated the serum levels of inflammatory mediators (IL-6 and calprotectin) and studied the correlation between them in healthy obese and normal weight adults. Methods: 33 healthy obese (HO) and 14 normal weight (NW) controls were included. Serum levels of IL-6 and calprotectin were estimated by ELISA. Abundances of Prevotella and γ-Proteobacteria were determined in stool using real time PCR. Results: IL-6 and calprotectin levels were significantly lower in HO than in NW (p=0.001, p=0.001 respectively) with significant negative correlation between IL-6 and body mass index (BMI) in HO (r=-0.438, p=0.011) and negative but statistically insignificant correlation with BMI in NW groups (r=-0.024, p=0.935). There was negative but statistically insignificant correlation between calprotectin and BMI in HO (r=-0.075, p=0.677) and NW (r=-0.381, p=0.179). Prevotella and γ-Proteobacteria abundances were higher in HO than in NW and insignificant (p=0.31, p=0.55 respectively) with significant positive correlation between Prevotella and γ-Proteobacteria abundance in HO (r=0.436, p=0.011), positive and insignificant correlations between abundance of Prevotella and levels of IL-6 in HO and NW (r=0.303, P=0.086 vs r=0.316, p=0.272 respectively). Conclusion: IL-6 and calprotectin have role in regulation of energy homeostasis. Elevated abundances of Prevotella and γ-Proteobacteria may be the primary shifts in gut microbiota of  HO.
Keywords
Prevotella; γ-Proteobacteria; IL-6; calprotectin; healthy obese
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