Citrus clementine peels essential oil exhibited anti-SARS-CoV-2 and its modulatory effect against cytokine storm: Evidence from in vitro and in silico studies
Asaad, G., Abdelhameed, M., Elraey, M., roshdy, W., Elgamal, A., Moemen, Y. (2022). Citrus clementine peels essential oil exhibited anti-SARS-CoV-2 and its modulatory effect against cytokine storm: Evidence from in vitro and in silico studies. EKB Journal Management System, 65(10), 419-427. doi: 10.21608/ejchem.2022.116657.5271
Gihan Farag Asaad; Mohamed Fayed Abdelhameed; Mohamed Elraey; wael hamed roshdy; Abdelbaset Elgamal; Yasmine S. Moemen. "Citrus clementine peels essential oil exhibited anti-SARS-CoV-2 and its modulatory effect against cytokine storm: Evidence from in vitro and in silico studies". EKB Journal Management System, 65, 10, 2022, 419-427. doi: 10.21608/ejchem.2022.116657.5271
Asaad, G., Abdelhameed, M., Elraey, M., roshdy, W., Elgamal, A., Moemen, Y. (2022). 'Citrus clementine peels essential oil exhibited anti-SARS-CoV-2 and its modulatory effect against cytokine storm: Evidence from in vitro and in silico studies', EKB Journal Management System, 65(10), pp. 419-427. doi: 10.21608/ejchem.2022.116657.5271
Asaad, G., Abdelhameed, M., Elraey, M., roshdy, W., Elgamal, A., Moemen, Y. Citrus clementine peels essential oil exhibited anti-SARS-CoV-2 and its modulatory effect against cytokine storm: Evidence from in vitro and in silico studies. EKB Journal Management System, 2022; 65(10): 419-427. doi: 10.21608/ejchem.2022.116657.5271

Citrus clementine peels essential oil exhibited anti-SARS-CoV-2 and its modulatory effect against cytokine storm: Evidence from in vitro and in silico studies

Egyptian Journal of Chemistry
Article 38, Volume 65, Issue 10, October 2022, Page 419-427  XML PDF (755.76 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.116657.5271
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Authors
Gihan Farag Asaad email orcid 1; Mohamed Fayed Abdelhameedorcid 2; Mohamed Elraey3; wael hamed roshdy4; Abdelbaset Elgamal5; Yasmine S. Moemen6
1Department of Pharmacology, National Research Centre
2Pharmacology department, medical division, national research centre
3National Research centre
4Central Public Health Laboratory, Ministry of Health and Population, Cairo, Egypt
5Department of Chemistry of Natural and microbial products, Pharmaceutical institute, National Research Centre, Dokki, Cairo, Egypt
6Clinical Pathology Department, National Liver Institute, Menoufia University, Egypt
Abstract
SARS CoV-2 gets over more than four million people all over the world. The challenges for developing vaccines in overwhelming pandemic situations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), developing and screening of unique antiviral agents are peremptorily necessitated. In this study, we aimed to identify the chemical constituents of Citrus clementine peel essential oil (CCPEO) and to investigate its activities as anti-SARS-CoV-2 and anti-inflammatory activities. The chemical profile of CCPEO was identified via Gas chromatography-mass spectrometry analysis (GC/MS). The in-vitro cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and the 50% cytotoxic concentration (CC50) of CCPEO was determined. The antiviral effect of citrus clementine extract was determined by plaque reduction assay. A geometry-based molecular docking approach (Patchdock) was performed to create docking modifications that result in good molecular shape complementarity. The antiviral effect of CCPEO was attributed to the downregulation of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) released from Huh7cells, and thus attenuating the SARSCoV-2 infection-associated cytokine storm in severe cases.
Keywords
citrus clementine; essential oil; SARS CoV-2; IL-6, TNFα; molecular docking
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