Isolation and identification of Enterococcus faecium from Meat products with special reference to some virulence and antibiotics resistance factors.
Younis, W., Mohamed, A., Abd Al-azeem, M., Mohamed, H. (2022). Isolation and identification of Enterococcus faecium from Meat products with special reference to some virulence and antibiotics resistance factors.. EKB Journal Management System, 5(1), 1-16. doi: 10.21608/svu.2022.88396.1137
Waleed Younis; Asmaa A Mohamed; Mohamed W Abd Al-azeem; Hams M.A Mohamed. "Isolation and identification of Enterococcus faecium from Meat products with special reference to some virulence and antibiotics resistance factors.". EKB Journal Management System, 5, 1, 2022, 1-16. doi: 10.21608/svu.2022.88396.1137
Younis, W., Mohamed, A., Abd Al-azeem, M., Mohamed, H. (2022). 'Isolation and identification of Enterococcus faecium from Meat products with special reference to some virulence and antibiotics resistance factors.', EKB Journal Management System, 5(1), pp. 1-16. doi: 10.21608/svu.2022.88396.1137
Younis, W., Mohamed, A., Abd Al-azeem, M., Mohamed, H. Isolation and identification of Enterococcus faecium from Meat products with special reference to some virulence and antibiotics resistance factors.. EKB Journal Management System, 2022; 5(1): 1-16. doi: 10.21608/svu.2022.88396.1137

Isolation and identification of Enterococcus faecium from Meat products with special reference to some virulence and antibiotics resistance factors.

SVU-International Journal of Veterinary Sciences
Article 1, Volume 5, Issue 1 - Serial Number 11, March 2022, Page 1-16  XML PDF (336.19 K)
Document Type: Research article
DOI: 10.21608/svu.2022.88396.1137
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Authors
Waleed Younis email ; Asmaa A Mohamed; Mohamed W Abd Al-azeem; Hams M.A Mohamed
microbiology department , faculty of veterinary medicine, south valley university
Abstract
Enterococcus faecium is one of the most superbug pathogen which cause many diseases in human which include wound infection and endocarditis. also, this microorganism show high resistant to common antibiotics which used in hospital especially Vancomycin so the purpose of this study is detection E.Faecium from meat products which can be achieved by isolation and identification of E.faecium and detection some virulence gene and antibiotic resistance gene by PCR. Biochemical and molecular studies were performed on seventeen unidentified gram-stain positive, catalase and oxidase negative, Enterococcus-like organisms recovered from hundred meat products samples. The result of Vitek-2 compact system identified the suspected isolates as E. faecium. Antibiotic susceptibility testing of the isolates indicated resistance to most antibiotics, including penicillin, ampicillin, amoxicillin and vancomycin. The molecular analysis indicated the presence of virulence genes such as hyl (30%), ace (30%) and cylA (90%), and antimicrobial resistance genes as followed vanA gene (50%) and blaZ gene (90%). In conclusion meat products considered a good source of infection by vancomycin resistant Enterococcus Faecium (VRE) which have potential risk on human being so hygienic measurement are required during meat processing.
Keywords
Keywords: Meat products; E. faecium; virulence genes; antimicrobial resistance genes
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