Green Spectrofluorimetric Technique for the Determination of Vildagliptin Through Quenching Lanthanide Luminescence in Pure, Pharmaceutical Dosage Form and Spiked Plasma
Mohamed, D., Abou El- alamin, M., Tubar, S. (2022). Green Spectrofluorimetric Technique for the Determination of Vildagliptin Through Quenching Lanthanide Luminescence in Pure, Pharmaceutical Dosage Form and Spiked Plasma. EKB Journal Management System, 6(1), 6-14. doi: 10.21608/aprh.2021.98458.1143
Dina Mohamed; Maha Abou El- alamin; Safaa Tubar. "Green Spectrofluorimetric Technique for the Determination of Vildagliptin Through Quenching Lanthanide Luminescence in Pure, Pharmaceutical Dosage Form and Spiked Plasma". EKB Journal Management System, 6, 1, 2022, 6-14. doi: 10.21608/aprh.2021.98458.1143
Mohamed, D., Abou El- alamin, M., Tubar, S. (2022). 'Green Spectrofluorimetric Technique for the Determination of Vildagliptin Through Quenching Lanthanide Luminescence in Pure, Pharmaceutical Dosage Form and Spiked Plasma', EKB Journal Management System, 6(1), pp. 6-14. doi: 10.21608/aprh.2021.98458.1143
Mohamed, D., Abou El- alamin, M., Tubar, S. Green Spectrofluorimetric Technique for the Determination of Vildagliptin Through Quenching Lanthanide Luminescence in Pure, Pharmaceutical Dosage Form and Spiked Plasma. EKB Journal Management System, 2022; 6(1): 6-14. doi: 10.21608/aprh.2021.98458.1143

Green Spectrofluorimetric Technique for the Determination of Vildagliptin Through Quenching Lanthanide Luminescence in Pure, Pharmaceutical Dosage Form and Spiked Plasma

Journal of Advanced Pharmacy Research
Article 2, Volume 6, Issue 1, January 2022, Page 6-14  XML PDF (1013.88 K)
Document Type: Research Article
DOI: 10.21608/aprh.2021.98458.1143
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Authors
Dina Mohamed email orcid ; Maha Abou El- alaminorcid ; Safaa Tubar
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, 11795, Cairo, Egypt
Abstract
Objectives: The objective of the proposed method is to develop a validated sensitive, selective, rapid, and low-cost spectrofluorimetric technique for the estimation of Vildagliptin depending on its quenching effect on the fluorescence intensity of Terbium (Tb3+). Methods: The proposed method involves quantitative fluorescence quenching of terbium measured at 477 nm after excitation at 239 nm. Various experimental conditions were investigated as pH, time of reaction, order of mixing of reagents, concentrations of Tb3+ and buffer. Results: Under optimum conditions, good linearity was obtained within the range 20.0-3000.0 ng/mL with correlation coefficients of 0.9997. LOD and LOQ were 1.8 ng/mL and 19.2 ng/mL, respectively. Conclusion: The proposed method accomplished greener parameters; accordingly, it is a green choice for the determination of Vildagliptin in bulk, pharmaceutical dosage form, and spiked plasma.
Keywords
spectroflorimetry; Terbium; Vildagliptin
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