Quantification of ketoprofen in film-coated tablets using hydrophilic interaction liquid chromatography-Ultraviolet spectrometry | ||||
| Egyptian Journal of Chemistry | ||||
| Article 15, Volume 65, Issue 9, September 2022, Page 147-153 PDF (284.89 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2022.113077.5138 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ali Amer Waheb 1; Ashraf S. Rasheed2; Mohammed J. M. Hassan3
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| 1Departments of Chemistry, College of Science, Mustansiriyah University, Baghdad, Iraq. | ||||
| 2Department of Chemistry, College of Science, University of Baghdad, Al-Jadriya campus, 10071 Baghdad, Iraq | ||||
| 3Departments of Chemistry, College of Science, Mustansiriyah University, Baghdad, Iraq | ||||
| Abstract | ||||
| A simple zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) method was developed and applied to film-coated tablets to determine ketoprofen. Separation was performed using gradient elution with a mixture of acetonitrile and acetate buffer at an Ultraviolet (UV) detection wavelength of 255 nm on self-constructed ZIC-HILIC-1 and ZIC-HILIC-4 columns. The hydrophilicity of ketoprofen was validated by examining its behaviour with varied acetate buffer, acetonitrile proportions, and pH values. The mechanism of separation is based on ketoprofen partitioning in the hydrophobic interaction. The two available approaches are a viable alternative to the present ketoprofen separation methods. The developed ZIC-HILIC methods had an excellent precision (0.61%), linear ranges of 0.05-10 ppm, and detection limits of 0.01-0.008 ppm with a coefficient of 0.9995 0.9997 for the ZIC-HILIC-1 and ZIC-HILIC-4 columns, respectively. The methods' findings were compared to the British Pharmacopoeia procedure using statistical testing, which revealed no difference in accuracy between the methods. | ||||
| Keywords | ||||
| ZIC-HILIC; Pharmaceutical formulation; ketoprofen; NSAIDs; HPLC | ||||
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