Study of TNF α gene polymorphism in Type 2 Diabetes | ||||
Minia Journal of Medical Research | ||||
Volume 31, Issue 4, October 2020, Page 67-71 PDF (249.7 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/mjmr.2022.217067 | ||||
View on SCiNiTO | ||||
Authors | ||||
Sahar H. El-Hin1; Amira T. Zaki Ahmed2; Eglal MS Hamed2; Yehia Z. Mahmoud2; Amel M. Kamal Eldin3; Hend M. Abdelghany4 | ||||
1Diabetes and Endocrinology unit, Department of Internal Medicine, Minia School of Medicine, Minia University, Egypt. | ||||
2Department of Internal Medicine, Minia School of Medicine, Minia University, Egypt. | ||||
3Department of Clinical Pathology, Minia School of Medicine, Minia University, Egypt. | ||||
4Department of Biochemistry, Minia School of Medicine, Minia University, Egypt. | ||||
Abstract | ||||
DM is one of the five principal causes of death universally. It is a growing heath problem of multifactorial origin, understanding its genetic background might help in early detection and disease prevention. Obesity, especially visceral adiposity, and physical inactivity are major risk factors for diabetes in Egypt. A 2010 World Health Organization (WHO) report indicated that 30.3% of Egyptian adults are obese. Egypt currently has the third highest prevalence of obesity in the Middle East and North Africa (MENA) region, after Saudi Arabia and United Arab Emirates. It has been reported that genetic variations in the promoter region regulate tumor necrosis factor-α (TNFα) production and transcription and, they influence susceptibility to inflammatory related diseases. Single nucleotide polymorphism studies in the promoter region of TNFα (_238) have suggested its role in increased insulin resistance. This is a prospective case control study aimed at detecting the impact of TNF α 238 G/A rs 361525 gene polymorphisms in T2DM in Egyptian population. It included 40 diabetic patients, and 40 apparently healthy unrelated volunteers. We underwent history taking, clinical examination with detection of BMI and W/H ratio, laboratory investigations, (finger prick test, 75 gram oral glucose tolerance test, HA1C, fasting and 2hPP blood glucose, and detection of TNF -238G/A gene polymorphisms that was performed by using PCR/RFLP method. In respect to the distribution of TNFα 238 G/A polymorphism genotypes: In patients with T2DM it was (GG genotype: 75%, AA genotype 5%, and GA genotype: 20%). In control it was (GG genotype: 90%, AA genotype: 2.5%, and GA genotype: 7.5%). | ||||
Keywords | ||||
TNFα G/A 238 gene polymorphism; Type2 Diabetes; obesity; insulin resistance | ||||
Statistics Article View: 71 PDF Download: 206 |
||||