MOLECULAR MARKERS DISTINGUISHING ENCYRTID (Hymenoptera: Encyrtidae) PARASITOIDS ATTACKING SOFT SCALE INSECTS (Hemiptera: Coccidae)
Abd-Rabou,, S., Refaat, M., Shalaby, H. (2009). MOLECULAR MARKERS DISTINGUISHING ENCYRTID (Hymenoptera: Encyrtidae) PARASITOIDS ATTACKING SOFT SCALE INSECTS (Hemiptera: Coccidae). EKB Journal Management System, 34(12), 11429-11440. doi: 10.21608/jppp.2009.217512
Sh. Abd-Rabou,; M. H. Refaat; H. H. Shalaby. "MOLECULAR MARKERS DISTINGUISHING ENCYRTID (Hymenoptera: Encyrtidae) PARASITOIDS ATTACKING SOFT SCALE INSECTS (Hemiptera: Coccidae)". EKB Journal Management System, 34, 12, 2009, 11429-11440. doi: 10.21608/jppp.2009.217512
Abd-Rabou,, S., Refaat, M., Shalaby, H. (2009). 'MOLECULAR MARKERS DISTINGUISHING ENCYRTID (Hymenoptera: Encyrtidae) PARASITOIDS ATTACKING SOFT SCALE INSECTS (Hemiptera: Coccidae)', EKB Journal Management System, 34(12), pp. 11429-11440. doi: 10.21608/jppp.2009.217512
Abd-Rabou,, S., Refaat, M., Shalaby, H. MOLECULAR MARKERS DISTINGUISHING ENCYRTID (Hymenoptera: Encyrtidae) PARASITOIDS ATTACKING SOFT SCALE INSECTS (Hemiptera: Coccidae). EKB Journal Management System, 2009; 34(12): 11429-11440. doi: 10.21608/jppp.2009.217512

MOLECULAR MARKERS DISTINGUISHING ENCYRTID (Hymenoptera: Encyrtidae) PARASITOIDS ATTACKING SOFT SCALE INSECTS (Hemiptera: Coccidae)

Journal of Plant Protection and Pathology
Article 3, Volume 34, Issue 12, December 2009, Page 11429-11440  XML PDF (756.04 K)
Document Type: Original Article
DOI: 10.21608/jppp.2009.217512
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Authors
Sh. Abd-Rabou,1; M. H. Refaat2; H. H. Shalaby1
1Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
2Department of Genetics, Faculty of Agriculture, Moshtohor, Benha University, Egypt
Abstract
      Reciprocal molecular markers were developed to distinguish the closely related parasitoid genera Encyrtus Latreille, Metaphycus Mercet and Paraceraptrocerus  Girault (Hymenoptera : Encyrtidae). These parasitoids are widely used in the biological control of soft scales and based upon morphology, it is extremely difficult to distinguish them. DNA extraction and RAPD-PCR were used to assess the amount of genetic variation within and among species at this locus. From parsimony analysis, populations of Encyrtus inflelix (Embleton), Metaphycus anneckei Guerrieri & Noyes and Paraceraptrocerus africanus Giralut were separated. Specific DNA markers of E. inflelix, P. africanus and M. anneckei at M.W. of (475.07, 429.60, 311.37bp < /em>) and (473.33bp), (700.0, 400.0 and 270.59bp < /em>) and, (918.79, 814.45, 771.44bp < /em>) for (AC07), (AE02), (B10) and (B07) primers, respectively were used.  Based upon the sequence variation among species, the present study appeared a simple molecular assay to rapidly and unambiguously distinguish E. inflelix, P. africanus and M. anneckei species.
Keywords
Encyrtidae; molecular markers; parasitoids and soft scale
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